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WB analysis of various sample lysates using GTX32552 DBC1 antibody. Dilution : 1:1000 Loading : 25microg per lane
WB analysis of various sample lysates using GTX32552 DBC1 antibody. Dilution : 1:1000 Loading : 25microg per lane
WB analysis of various sample lysates using GTX32552 DBC1 antibody. Dilution : 1:1000 Loading : 25microg per lane

DBC1 antibody

GTX32552
GeneTex
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetCCAR2
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Overview

  • Supplier
    GeneTex
  • Product Name
    DBC1 antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500 - 1:2000. ICC/IF: 1:50 - 1:200. IHC-P: 1:50 - 1:200. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID57805
  • Target name
    CCAR2
  • Target description
    cell cycle and apoptosis regulator 2
  • Target synonyms
    DBC-1, DBC1, KIAA1967, NET35, p30 DBC, p30DBC, cell cycle and apoptosis regulator protein 2, cell division cycle and apoptosis regulator protein 2, deleted in breast cancer 1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ8N163
  • Protein Name
    Cell cycle and apoptosis regulator protein 2
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

References

  • Arai M, Ochi H, Sunamura S, et al. A Novel Long Noncoding RNA in Osteocytes Regulates Bone Formation through the Wnt/β-Catenin Signaling Pathway. Int J Mol Sci. 2023,24(17). doi: 10.3390/ijms241713633
    Read this paper

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Figure 1. Western blot analysis of DBC-1/CCAR2 using anti-DBC-1/CCAR2 antibody (A03412-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates, Lane 2: monkey COS-7 whole cell lysates, Lane 3: human RT4 whole cell lysates, Lane 4: human K562 whole cell lysates, Lane 5: human HEL whole cell lysates, Lane 6: rat brain tissue lysates, Lane 7: mouse brain tissue lysates, Lane 8: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DBC-1/CCAR2 antigen affinity purified polyclonal antibody (Catalog # A03412-1) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for DBC-1/CCAR2 at approximately 130 kDa. The expected band size for DBC-1/CCAR2 is at 130 kDa.
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