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Immunohistochemistry of paraffin-embedded human testis tissue using CSB-PA887010LA01HU at dilution of 1:100
Immunohistochemistry of paraffin-embedded human testis tissue using CSB-PA887010LA01HU at dilution of 1:100
Immunohistochemistry of paraffin-embedded human testis tissue using CSB-PA887010LA01HU at dilution of 1:100

DBF4 Antibody

CSB-PA887010LA01HU
Cusabio
ApplicationsELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetDBF4
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Overview

  • Supplier
    Cusabio
  • Product Name
    DBF4 Antibody
  • Delivery Days Customer
    20
  • Applications
    ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID10926
  • Target name
    DBF4
  • Target description
    DBF4-CDC7 kinase regulatory subunit
  • Target synonyms
    ASK, CHIF, DBF4A, ZDBF1, protein DBF4 homolog A, DBF4 homolog, DBF4 zinc finger A, DBF4-type zinc finger-containing protein 1, activator of S phase kinase, chiffon homolog A, regulatory subunit of DDK, zinc finger, DBF-type containing 1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9UBU7
  • Protein Name
    Protein DBF4 homolog A
  • Scientific Description
    Regulatory subunit for CDC7 which activates its kinase activity thereby playing a central role in DNA replication and cell proliferation. Required for progression of S phase. The complex CDC7-DBF4A selectively phosphorylates MCM2 subunit at Ser-40 and Ser-53 and then is involved in regulating the initiation of DNA replication during cell cycle.
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

FAQ

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Figure 1. Western blot analysis of DBF4 using anti-DBF4 antibody (A01348-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human SH-SY5Y whole cell lysates, Lane 3: rat PC-12 whole cell lysates, Lane 4: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DBF4 antigen affinity purified polyclonal antibody (Catalog # A01348-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for DBF4 at approximately 80 kDa. The expected band size for DBF4 is at 80 kDa.
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