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Flow cytometric analysis of DBF4 was done on F9 cells. The cells were fixed, permeabilized and stained with the primary antibody (bsm-54705R, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Flow cytometric analysis of DBF4 was done on F9 cells. The cells were fixed, permeabilized and stained with the primary antibody (bsm-54705R, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Flow cytometric analysis of DBF4 was done on F9 cells. The cells were fixed, permeabilized and stained with the primary antibody (bsm-54705R, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

DBF4 Monoclonal Antibody

BSM-54705R
Bioss Antibodies
ApplicationsFlow Cytometry, Western Blot
DownloadDatasheet
Product group Antibodies
TargetDBF4
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Overview

  • Supplier
    Bioss Antibodies
  • Product Name
    DBF4 Monoclonal Antibody
  • Delivery Days Customer
    16
  • Applications
    Flow Cytometry, Western Blot
  • Applications Supplier
    WB(1:300-5000), FCM(1:20-100)
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    5H0
  • Concentration
    1 ug/ul
  • Conjugate
    Unconjugated
  • Gene ID10926
  • Target name
    DBF4
  • Target description
    DBF4 zinc finger
  • Target synonyms
    activator of S phase kinase; ASK; CHIF; chiffon homolog A; DBF4 homolog; DBF4 zinc finger A; DBF4A; DBF4-type zinc finger-containing protein 1; protein DBF4 homolog A; ZDBF1; zinc finger, DBF-type containing 1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9UBU7
  • Protein Name
    Protein DBF4 homolog A
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of DBF4 using anti-DBF4 antibody (A01348-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human SH-SY5Y whole cell lysates, Lane 3: rat PC-12 whole cell lysates, Lane 4: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DBF4 antigen affinity purified polyclonal antibody (Catalog # A01348-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for DBF4 at approximately 80 kDa. The expected band size for DBF4 is at 80 kDa.
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