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Western Blot Positive WB detected in: 293 whole cell lysate, Mouse kidney tissue, Mouse liver tissue All lanes: DBT antibody at 4.3microg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 54 kDa Observed band size: 54 kDa
Western Blot Positive WB detected in: 293 whole cell lysate, Mouse kidney tissue, Mouse liver tissue All lanes: DBT antibody at 4.3microg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 54 kDa Observed band size: 54 kDa
Western Blot Positive WB detected in: 293 whole cell lysate, Mouse kidney tissue, Mouse liver tissue All lanes: DBT antibody at 4.3microg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 54 kDa Observed band size: 54 kDa

DBT Antibody

CSB-PA006530LA01HU
Cusabio
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityHuman, Mouse
TargetDBT
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Overview

  • Supplier
    Cusabio
  • Product Name
    DBT Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID1629
  • Target name
    DBT
  • Target description
    dihydrolipoamide branched chain transacylase E2
  • Target synonyms
    BCATE2, BCKAD-E2, BCKADE2, BCKDH-E2, BCOADC-E2, E2, E2B, lipoamide acyltransferase component of branched-chain alpha-keto acid dehydrogenase complex, mitochondrial, 52 kDa mitochondrial autoantigen of primary biliary cirrhosis, BCKAD E2 subunit, E2 component of branched chain alpha-keto acid dehydrogenase complex, branched chain 2-oxo-acid dehydrogenase complex component E2, branched chain acyltransferase, E2 component, branched-chain alpha-keto acid dehydrogenase complex component E2, dihydrolipoamide acetyltransferase component of branched-chain alpha-keto acid dehydrogenase complex, dihydrolipoyl transacylase, dihydrolipoyllysine-residue (2-methylpropanoyl)transferase, lipoamide acyltransferase component of mitochondrial branched-chain alpha-keto acid dehydrogenase complex, mitochondrial branched chain alpha-keto acid dehydrogenase transacylase subunit (E2b)
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP11182
  • Protein Name
    Lipoamide acyltransferase component of branched-chain alpha-keto acid dehydrogenase complex, mitochondrial
  • Scientific Description
    The branched-chain alpha-keto dehydrogenase complex catalyzes the overall conversion of alpha-keto acids to acyl-CoA and CO(2). It contains multiple copies of three enzymatic components: branched-chain alpha-keto acid decarboxylase (E1), lipoamide acyltransferase (E2) and lipoamide dehydrogenase (E3). Within this complex, the catalytic function of this enzyme is to accept, and to transfer to coenzyme A, acyl groups that are generated by the branched-chain alpha-keto acid decarboxylase component.
  • Reactivity
    Human, Mouse
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of DBT using anti-DBT antibody (A01145-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human SiHa whole cell lysates, Lane 2: human A431 whole cell lysates, Lane 3: human Hacat whole cell lysates, Lane 4: human Jurkat whole cell lysates, Lane 5: rat stomach tissue lysates, Lane 6: rat kidney tissue lysates, Lane 7: mouse stomach tissue lysates, Lane 8: mouse kidney tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DBT antigen affinity purified polyclonal antibody (A01145-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for DBT at approximately 53 kDa. The expected band size for DBT is at 53 kDa.
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