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Inhibition of DCAF1 auto-ubiquitination activity by Methylated Ubiquitin Recombinant.
Inhibition of DCAF1 auto-ubiquitination activity by Methylated Ubiquitin Recombinant.
Inhibition of DCAF1 auto-ubiquitination activity by Methylated Ubiquitin Recombinant.

DCAF1 Intrachain TR-FRET Assay Kit

Research Use Only
82517
BPS Bioscience
Product group Assays
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Overview

  • Supplier
    BPS Bioscience
  • Product Name
    DCAF1 Intrachain TR-FRET Assay Kit
  • Delivery Days Customer
    7
  • Certification
    Research Use Only
  • Scientific Description
    The DCAF1 Intrachain TR-FRET Assay Kit is a homogeneous, sensitive TR-FRET (Time-Resolved Fluorescence Resonance Energy Transfer) assay kit designed to measure DCAF1 (DDB1 and CUL4-associated Factor 1) complex auto-ubiquitination activity. It utilizes a mix of donor and acceptor labeled ubiquitin to complete the TR-FRET pairing. This assay measures poly-ubiquitination since both the TR-FRET donor and acceptor are incorporated into poly-ubiquitin chains formed on DCAF1. The kit contains enough purified DCAF1/Rbx1 (M5I)/DDB1/CUL4A Complex, purified UBE1 and UbcH5b, detection reagents and assay buffer for 400 reactions.
  • Storage Instruction
    See manual
  • UNSPSC
    41116133