Inhibition of DCAF1 auto-ubiquitination activity by Methylated Ubiquitin Recombinant.
DCAF1 Intrachain TR-FRET Assay Kit
82517
Product group Assays
Overview
- SupplierBPS Bioscience
- Product NameDCAF1 Intrachain TR-FRET Assay Kit
- Delivery Days Customer7
- CertificationResearch Use Only
- Scientific DescriptionThe DCAF1 Intrachain TR-FRET Assay Kit is a homogeneous, sensitive TR-FRET (Time-Resolved Fluorescence Resonance Energy Transfer) assay kit designed to measure DCAF1 (DDB1 and CUL4-associated Factor 1) complex auto-ubiquitination activity. It utilizes a mix of donor and acceptor labeled ubiquitin to complete the TR-FRET pairing. This assay measures poly-ubiquitination since both the TR-FRET donor and acceptor are incorporated into poly-ubiquitin chains formed on DCAF1. The kit contains enough purified DCAF1/Rbx1 (M5I)/DDB1/CUL4A Complex, purified UBE1 and UbcH5b, detection reagents and assay buffer for 400 reactions.
- Storage InstructionSee manual
- UNSPSC41116133