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Western blot All lanes: DCN antibody at 2microg/ml Lane 1: Mouse heart tissue Lane 2: HepG2 whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 40, 28, 24, 20, 9 kDa Observed band size: 40 kDa
Western blot All lanes: DCN antibody at 2microg/ml Lane 1: Mouse heart tissue Lane 2: HepG2 whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 40, 28, 24, 20, 9 kDa Observed band size: 40 kDa
Western blot All lanes: DCN antibody at 2microg/ml Lane 1: Mouse heart tissue Lane 2: HepG2 whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 40, 28, 24, 20, 9 kDa Observed band size: 40 kDa

DCN Antibody

CSB-PA10289A0RB
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
TargetDCN
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Overview

  • Supplier
    Cusabio
  • Product Name
    DCN Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID1634
  • Target name
    DCN
  • Target description
    decorin
  • Target synonyms
    bone proteoglycan II; CSCD; decorin; dermatan sulphate proteoglycans II; DSPG2; PG40; PGII; PGS2; proteoglycan core protein; SLRR1B; small leucine-rich protein 1B
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP07585
  • Protein Name
    Decorin
  • Scientific Description
    May affect the rate of fibrils formation.
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    12352203

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Lane 1: Human U2os cell lysates; Lane 2: Human Huvec cell lysates probed with Decorin Polyclonal Antibody, Unconjugated (bs-1695R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
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Figure 1. Western blot analysis of Decorin using anti-Decorin antibody (PB9174). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human MCF-7 whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human HepG2 whole cell lysates, Lane 4: human SW620 whole cell lysates, Lane 5: rat liver tissue lysates, Lane 6: rat RH35 whole cell lysates, Lane 7: rat PC-12 whole cell lysates, Lane 8: rat C6 whole cell lysates, Lane 9: mouse thymus tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Decorin antigen affinity purified polyclonal antibody (Catalog # PB9174) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. Specific bands were detected for Decorin at approximately 40-50KD and 80-140KD. The expected band sizes for Decorin are at 40-50KD and 80-140KD.
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