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ProductsBack/Decorin Polyclonal Antibody
Lane 1: Human U2os cell lysates; Lane 2: Human Huvec cell lysates probed with Decorin Polyclonal Antibody, Unconjugated (bs-1695R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: Human U2os cell lysates; Lane 2: Human Huvec cell lysates probed with Decorin Polyclonal Antibody, Unconjugated (bs-1695R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: Human U2os cell lysates; Lane 2: Human Huvec cell lysates probed with Decorin Polyclonal Antibody, Unconjugated (bs-1695R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Decorin Polyclonal Antibody

BS-1695R
Bioss
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetDCN
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Overview

  • Supplier
    Bioss
  • Product Name
    Decorin Polyclonal Antibody
  • Delivery Days Customer
    16
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    WB(1:300-5000), ELISA(1:500-1000), IHC-P(1:200-400)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 ug/ul
  • Conjugate
    Unconjugated
  • Gene ID1634
  • Target name
    DCN
  • Target description
    decorin
  • Target synonyms
    CSCD, DSPG2, PG40, PGII, PGS2, SLRR1B, decorin, bone proteoglycan II, dermatan sulphate proteoglycans II, proteoglycan core protein, small leucine-rich protein 1B
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP07585
  • Protein Name
    Decorin
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

References

  • Shibata S, Amano H, Nagayama M, et al. Immunohistochemical and ultrastructural evaluation of matrix components in mandibular condylar cartilage in comparison with growth plate cartilage in cartilage calcification insufficient rats. Anat Sci Int. 2020,95(1):54-66. doi: 10.1007/s12565-019-00493-3
    Read this paper
  • Li X, Qiu J, Liu H, et al. Long-term thermal manipulation in the late incubation period can inhibit breast muscle development by activating endoplasmic reticulum stress in duck (Anasplatyrhynchos domestica). J Therm Biol. 2017,70(Pt B):37-45. doi: 10.1016/j.jtherbio.2017.10.008
    Read this paper
  • Li G, Li M, Liang X, et al. Identifying DCN and HSPD1 as Potential Biomarkers in Colon Cancer Using 2D-LC-MS/MS Combined with iTRAQ Technology. J Cancer. 2017,8(3):479-489. doi: 10.7150/jca.17192
    Read this paper
  • Peng JY, Gao KX, Xin HY, et al. Molecular cloning, expression analysis, and function of decorin in goat ovarian granulosa cells. Domest Anim Endocrinol. 2016,57:108-16. doi: 10.1016/j.domaniend.2016.05.006
    Read this paper

Datasheet

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Figure 1. Western blot analysis of Decorin using anti-Decorin antibody (PB9174). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human MCF-7 whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human HepG2 whole cell lysates, Lane 4: human SW620 whole cell lysates, Lane 5: rat liver tissue lysates, Lane 6: rat RH35 whole cell lysates, Lane 7: rat PC-12 whole cell lysates, Lane 8: rat C6 whole cell lysates, Lane 9: mouse thymus tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Decorin antigen affinity purified polyclonal antibody (Catalog # PB9174) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. Specific bands were detected for Decorin at approximately 40-50KD and 80-140KD. The expected band sizes for Decorin are at 40-50KD and 80-140KD.
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