More than 130 suppliers
ISO certified
In-house tech support
we Connect you
Bio-Connect
ProductsBack/F2 Polyclonal Antibody
Paraformaldehyde-fixed, paraffin embedded Human liver cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with DCP Polyclonal Antibody, Unconjugated (bs-1037R) at 1:400 overnight at 4°C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Human liver cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with DCP Polyclonal Antibody, Unconjugated (bs-1037R) at 1:400 overnight at 4°C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Human liver cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with DCP Polyclonal Antibody, Unconjugated (bs-1037R) at 1:400 overnight at 4°C, DAB staining.

F2 Polyclonal Antibody

BS-1037R
Bioss
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetF2
Sign in to order and to see your custom pricing.
Large volume orders?
Order with a bulk request
More than 130 suppliers
ISO certified
In-house tech support

Overview

  • Supplier
    Bioss
  • Product Name
    F2 Polyclonal Antibody
  • Delivery Days Customer
    16
  • Applications
    Flow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    WB(1:300-5000), ELISA(1:500-1000), FCM(1:20-100), IHC-P(1:200-400), IHC-F(1:100-500), IF(IHC-P)(1:50-200), IF(IHC-F)(1:50-200), IF(ICC)(1:50-200)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 ug/ul
  • Conjugate
    Unconjugated
  • Gene ID2147
  • Target name
    F2
  • Target description
    coagulation factor II, thrombin
  • Target synonyms
    PT, RPRGL2, THPH1, prothrombin, prepro-coagulation factor II, prothrombin B-chain, thrombin factor II
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP00734
  • Protein Name
    Prothrombin
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

Datasheet

Download Datasheet

FAQ

Go to our frequently asked questions

Related products

Western Blot analysis of Jurkat cells using Prothrombin Polyclonal Antibody
Product group Antibodies
Cusabio
F2 AntibodyCSB-PA004840
ApplicationsWestern Blot, ELISA
ReactivityHuman, Mouse, Rat
TargetF2
  • SizePrice
Product group Antibodies
MedChem Express
F2 Antibody(Rabbit)HY-P81243
TargetF2
  • SizePrice
Product group Antibodies
GeneTex
Prothrombin antibodyGTX101199
ApplicationsWestern Blot
TargetF2
  • SizePrice
Product group Antibodies
Biosynth
Thrombin antibody20R-1363
  • SizePrice
Anti-F2 Antibody
Product group Antibodies
Antibodies.com
Anti-F2 AntibodyA47288
ApplicationsImmunoHistoChemistry
  • SizePrice
Product group Antibodies
RayBiotech
Anti-F2 Antibody144-62315
ApplicationsImmunoFluorescence, Western Blot
TargetF2
  • SizePrice
Figure 1. Western blot analysis of Prothrombin using anti-Prothrombin antibody (A00044). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human plasma lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Prothrombin antigen affinity purified polyclonal antibody (Catalog # A00044) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Prothrombin at approximately 85KD. The expected band size for Prothrombin is at 70KD.
Product group Antibodies
Boster Bio
Anti-Prothrombin/F2 Antibody Picoband(r)A00044-CARRIER-FREE
ApplicationsFlow Cytometry, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry
TargetF2
  • SizePrice
Product group Antibodies
Absolute Antibody
Anti-prothrombin [POmAb], Mouse IgG1, kappaAB04499-1.1
ApplicationsOther Application
TargetF2
  • SizePrice
Immunohistochemistry analysis in human liver and skeletal muscle tissues using HPA051476 antibody. Corresponding F2 RNA-seq data are presented for the same tissues.
Product group Antibodies
Atlas Antibodies
Anti-F2 AntibodyHPA051476
ApplicationsWestern Blot, ImmunoHistoChemistry
ReactivityHuman
TargetF2
  • SizePrice
Product group Antibodies
Biomatik
F2 Polyclonal AntibodyCAC08415
ApplicationsImmunoFluorescence, Western Blot, ELISA
TargetF2
  • SizePrice