Bio-Connect
Western Blot analysis of Jurkat cells using Prothrombin Polyclonal Antibody
Western Blot analysis of Jurkat cells using Prothrombin Polyclonal Antibody
Western Blot analysis of Jurkat cells using Prothrombin Polyclonal Antibody

F2 Antibody

CSB-PA004840
Cusabio
ApplicationsWestern Blot, ELISA
Product group Antibodies
TargetF2
Sign in to order and to see your custom pricing.
Large volume orders?
Order with a bulk request

Overview

  • Supplier
    Cusabio
  • Product Name
    F2 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID2147
  • Target name
    F2
  • Target description
    coagulation factor II, thrombin
  • Target synonyms
    prepro-coagulation factor II; prothrombin; prothrombin B-chain; PT; RPRGL2; THPH1; thrombin factor II
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP00734
  • Protein Name
    Prothrombin
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    12352203

Related products

Product group Antibodies
Biomatik
ApplicationsImmunoFluorescence, Western Blot, ELISA
TargetF2
  • SizePrice
Paraformaldehyde-fixed, paraffin embedded Human liver cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with DCP Polyclonal Antibody, Unconjugated (bs-1037R) at 1:400 overnight at 4°C, DAB staining.
Product group Antibodies
Bioss Antibodies
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
TargetF2
  • SizePrice
Product group Antibodies
MedChem Express
F2 AntibodyHY-P81243
TargetF2
  • SizePrice
Product group Antibodies
GeneTex
ApplicationsWestern Blot
TargetF2
  • SizePrice
Immunohistochemistry analysis in human liver and skeletal muscle tissues using HPA051476 antibody. Corresponding F2 RNA-seq data are presented for the same tissues.
Product group Antibodies
Atlas Antibodies
ApplicationsWestern Blot, ImmunoHistoChemistry
TargetF2
  • SizePrice
Product group Antibodies
RayBiotech
ApplicationsImmunoFluorescence, Western Blot
TargetF2
  • SizePrice
Product group Antibodies
Biosynth
  • SizePrice
Product group Antibodies
LifeSpan BioSciences
ApplicationsWestern Blot, ELISA
TargetF2
  • SizePrice
Figure 1. Western blot analysis of Prothrombin using anti-Prothrombin antibody (A00044). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human plasma lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Prothrombin antigen affinity purified polyclonal antibody (Catalog # A00044) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Prothrombin at approximately 85KD. The expected band size for Prothrombin is at 70KD.
Product group Antibodies
Boster Bio
ApplicationsFlow Cytometry, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry
TargetF2
  • SizePrice
Anti-F2 Antibody
Product group Antibodies
Antibodies.com
ApplicationsImmunoHistoChemistry
  • SizePrice