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Western Blot analysis of Jurkat cells using Prothrombin Polyclonal Antibody
Western Blot analysis of Jurkat cells using Prothrombin Polyclonal Antibody
Western Blot analysis of Jurkat cells using Prothrombin Polyclonal Antibody

F2 Antibody

CSB-PA004840
Cusabio
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetF2
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Overview

  • Supplier
    Cusabio
  • Product Name
    F2 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID2147
  • Target name
    F2
  • Target description
    coagulation factor II, thrombin
  • Target synonyms
    PT, RPRGL2, THPH1, prothrombin, prepro-coagulation factor II, prothrombin B-chain, thrombin factor II
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP00734
  • Protein Name
    Prothrombin
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of Prothrombin using anti-Prothrombin antibody (A00044). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human plasma lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Prothrombin antigen affinity purified polyclonal antibody (Catalog # A00044) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Prothrombin at approximately 85KD. The expected band size for Prothrombin is at 70KD.
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