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Western Blot Positive WB detected in: Mouse lung tissue, Mouse kidney tissue All lanes: DDAH2 antibody at 2.5ug/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 30 kDa Observed band size: 30, 50 kDa
Western Blot Positive WB detected in: Mouse lung tissue, Mouse kidney tissue All lanes: DDAH2 antibody at 2.5ug/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 30 kDa Observed band size: 30, 50 kDa
Western Blot Positive WB detected in: Mouse lung tissue, Mouse kidney tissue All lanes: DDAH2 antibody at 2.5ug/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 30 kDa Observed band size: 30, 50 kDa

DDAH2 Antibody

CSB-PA006580LA01HU
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse
TargetDDAH2
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Overview

  • Supplier
    Cusabio
  • Product Name
    DDAH2 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID23564
  • Target name
    DDAH2
  • Target description
    DDAH family member 2, ADMA-independent
  • Target synonyms
    DDAH, DDAHII, G6a, HEL-S-277, NG30, putative hydrolase DDAH2, S-phase protein, dimethylargininase-2, dimethylarginine dimethylaminohydrolase 2, dimethylarginine dimethylaminohydrolase II, epididymis secretory protein Li 277, inactive N(G),N(G)-dimethylarginine dimethylaminohydrolase 2, inactive dimethylarginine dimethylaminohydrolase 2, n(G),N(G)-dimethylarginine dimethylaminohydrolase 2, testis tissue sperm-binding protein Li 54e
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO95865
  • Protein Name
    N(G),N(G)-dimethylarginine dimethylaminohydrolase 2
  • Scientific Description
    Hydrolyzes N(G),N(G)-dimethyl-L-arginine (ADMA) and N(G)-monomethyl-L-arginine (MMA) which act as inhibitors of NOS. Has therefore a role in the regulation of nitric oxide generation.
  • Reactivity
    Human, Mouse
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

FAQ

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Figure 1. Western blot analysis of DDAH2 using anti-DDAH2 antibody (PB10001). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat lung tissue lysates, Lane 2: mouse lung tissue lysates, Lane 3: human placenta tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DDAH2 antigen affinity purified polyclonal antibody (Catalog # PB10001) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for DDAH2 at approximately 24 kDa. The expected band size for DDAH2 is at 24 kDa.
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