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ProductsBack/Goat anti-DDAH2

Goat anti-DDAH2

EB05277
Everest Biotech
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA
Product group Antibodies
TargetDDAH2
100 ug
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Overview

  • Supplier
    Everest Biotech
  • Product Name
    Goat anti-DDAH2 Antibody
  • Delivery Days Customer
    5
  • Application Supplier Note
    Immunofluorescence: Strong expression of the protein seen in the cytoplasm of A431 cells. Recommended concentration: 10microg/ml. Flow Cytometry: Flow cytometric analysis of A431 cells. Recommended concentration: 10ug/ml.
  • Applications
    Flow Cytometry, ImmunoFluorescence, Western Blot, ELISA
  • Applications Supplier
    Pep-ELISA, WB, IF, FC
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5 mg/ml
  • Gene ID23564
  • Target name
    DDAH2
  • Target description
    DDAH family member 2, ADMA-independent
  • Target synonyms
    DDAH, DDAHII, G6a, HEL-S-277, NG30, putative hydrolase DDAH2, S-phase protein, dimethylargininase-2, dimethylarginine dimethylaminohydrolase 2, dimethylarginine dimethylaminohydrolase II, epididymis secretory protein Li 277, inactive N(G),N(G)-dimethylarginine dimethylaminohydrolase 2, inactive dimethylarginine dimethylaminohydrolase 2, n(G),N(G)-dimethylarginine dimethylaminohydrolase 2, testis tissue sperm-binding protein Li 54e
  • Host
    Goat
  • Scientific Description
    RefSeq number(s): NP_039268.1. Purification: Antigen affinity purified. Names and symbols: DDAH2; dimethylarginine dimethylaminohydrolase 2; DDAH; G6a; NG30; DDAHII; dimethylarginine dimethylaminohydrolase II; NG-dimethylarginine dimethylamino hydrolase homolog; OTTHUMP00000062666; DADB-110M10.5; OTTHUMP00000029307; OTTHUMP00000174406; alternat
  • Reactivity Supplier
    Human, Mouse, Rat, Dog, Cow
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of DDAH2 using anti-DDAH2 antibody (PB10001). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat lung tissue lysates, Lane 2: mouse lung tissue lysates, Lane 3: human placenta tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DDAH2 antigen affinity purified polyclonal antibody (Catalog # PB10001) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for DDAH2 at approximately 24 kDa. The expected band size for DDAH2 is at 24 kDa.
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