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DDB2 Antibody (YA2127, Rabbit)

HY-P82382
MedChem Express
TargetDDB2
Product group Antibodies
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Overview

  • Supplier
    MedChem Express
  • Product Name
    DDB2 Antibody (YA2127, Rabbit)
  • Delivery Days Customer
    5
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Gene ID1643
  • Target name
    DDB2
  • Target description
    damage specific DNA binding protein 2
  • Target synonyms
    DDBB, UV-DDB2, XPE, DNA damage-binding protein 2, DDB p48 subunit, UV-damaged DNA-binding protein 2, damage-specific DNA binding protein 2, 48kDa, xeroderma pigmentosum group E protein
  • Host
    Rabbit
  • Isotype
    IgG
  • Scientific Description
    DDB2 Antibody (YA2127) is a rabbit-derived non-conjugated IgG antibody (Clone NO.: YA2127), targeting DDB2, with a predicted molecular weight of 48 kDa (observed band size: 45 kDa). DDB2 Antibody (YA2127) can be used for WB, IHC-P, ICC/IF, FC experiment in human background.
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of DDB2 using anti-DDB2 antibody (A01430-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human A431 whole cell lysates, Lane 3: human Raji whole cell lysates, Lane 4: human HL-60 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DDB2 antigen affinity purified polyclonal antibody (Catalog # A01430-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for DDB2 at approximately 48 kDa. The expected band size for DDB2 is at 48 kDa.
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