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ProductsBack/DDR2 (3B11E4)

DDR2 (3B11E4)

SC-81707
Santa Cruz Biotechnology
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetDDR2
200 ug
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Overview

  • Supplier
    Santa Cruz Biotechnology
  • Product Name
    DDR2 (3B11E4)
  • Delivery Days Customer
    9
  • Applications
    ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Gene ID4921
  • Target name
    DDR2
  • Target description
    discoidin domain receptor tyrosine kinase 2
  • Target synonyms
    MIG20a, NTRKR3, TKT, TYRO10, WRCN, discoidin domain-containing receptor 2, CD167 antigen-like family member B, cell migration-inducing protein 20, discoidin domain receptor 2, discoidin domain receptor family, member 2, discoidin domain-containing receptor tyrosine kinase 2, hydroxyaryl-protein kinase, migration-inducing gene 16 protein, neurotrophic tyrosine kinase receptor related 3, receptor protein-tyrosine kinase TKT, tyrosine-protein kinase TYRO10
  • Host
    Mouse
  • Isotype
    IgG2a
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of DDR2 using anti-DDR2 antibody (A01698-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HT1080 whole cell lysates, Lane 2: human K562 whole cell lysates, Lane 3: human Hela whole cell lysates, Lane 4: rat C6 whole cell lysates, Lane 5: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DDR2 antigen affinity purified polyclonal antibody (Catalog # A01698-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for DDR2 at approximately 120 kDa. The expected band size for DDR2 is at 98 kDa.
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