![Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with DDX3 antibody [N3C2], Internal (GTX110614) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with DDX3 antibody [N3C2], Internal (GTX110614) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX110614/GTX110614_43250_20180615_WB_w_23060500_949.webp)
Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with DDX3 antibody [N3C2], Internal (GTX110614) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
DDX3 antibody [N3C2], Internal
GTX110614
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse
TargetDDX3X
Overview
- SupplierGeneTex
- Product NameDDX3 antibody [N3C2], Internal
- Delivery Days Customer9
- Application Supplier NoteWB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration0.7 mg/ml
- ConjugateUnconjugated
- Gene ID1654
- Target nameDDX3X
- Target descriptionDEAD-box helicase 3 X-linked
- Target synonymsCAP-Rf, DBX, DDX14, DDX3, HLP2, MRX102, MRXSSB, ATP-dependent RNA helicase DDX3X, DEAD (Asp-Glu-Ala-Asp) box helicase 3, X-linked, DEAD (Asp-Glu-Ala-Asp) box polypeptide 3, X-linked, DEAD box protein 3, X-chromosomal, DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 3, DEAD/H box-3, helicase-like protein 2
- HostRabbit
- IsotypeIgG
- Protein IDO00571
- Protein NameATP-dependent RNA helicase DDX3X
- ReactivityHuman, Mouse
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC41116161
![DDX3 antibody [N3C2], Internal detects DDX3 protein at cytoplasm and nucleus by immunofluorescent analysis. Sample: SK-N-SH cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: DDX3 stained by DDX3 antibody [N3C2], Internal (GTX110614) diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar= 10 μm. DDX3 antibody [N3C2], Internal detects DDX3 protein at cytoplasm and nucleus by immunofluorescent analysis. Sample: SK-N-SH cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: DDX3 stained by DDX3 antibody [N3C2], Internal (GTX110614) diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar= 10 μm.](https://www.genetex.com/upload/website/prouct_img/normal/GTX110614/GTX110614_43250_20181115_ICC_IF_w_23060500_190.webp)

![U87-MG whole cell and cytoplasm extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with DDX3 antibody [N3C2], Internal (GTX110614) diluted at 1:2000. U87-MG whole cell and cytoplasm extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with DDX3 antibody [N3C2], Internal (GTX110614) diluted at 1:2000.](https://www.genetex.com/upload/website/prouct_img/normal/GTX110614/GTX110614_40037_20160505_WB_Fraction_w_23060500_692.webp)

![DDX3 antibody [N3C2], Internal detects DDX3 protein at cytoplasm and nucleus by immunohistochemical analysis. Sample: Paraffin-embedded human breast carcinoma. DDX3 stained by DDX3 antibody [N3C2], Internal (GTX110614) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min DDX3 antibody [N3C2], Internal detects DDX3 protein at cytoplasm and nucleus by immunohistochemical analysis. Sample: Paraffin-embedded human breast carcinoma. DDX3 stained by DDX3 antibody [N3C2], Internal (GTX110614) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](https://www.genetex.com/upload/website/prouct_img/normal/GTX110614/GTX110614_43964_20200814_IHC-P_w_23060500_491.webp)
![Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with DDX3 antibody [N3C2], Internal (GTX110614) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced. Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with DDX3 antibody [N3C2], Internal (GTX110614) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](https://www.genetex.com/upload/website/prouct_img/normal/GTX110614/GTX110614_43964_20200529_WB_23103019_170.webp)







