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ProductsBack/Death receptor 5 (DR5) Polyclonal Antibody

Death receptor 5 (DR5) Polyclonal Antibody

CAU27048
Biomatik
ApplicationsImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
ReactivityMouse
TargetTnfrsf10b
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Overview

  • Supplier
    Biomatik
  • Product Name
    Death receptor 5 (DR5) Polyclonal Antibody
  • Delivery Days Customer
    12
  • Applications
    ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
  • Applications Supplier
    WB; IHC; ICC; IP.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID21933
  • Target name
    Tnfrsf10b
  • Target description
    tumor necrosis factor receptor superfamily, member 10b
  • Target synonyms
    DR5, KILLER, Ly98, MK, TRAILR2, TRICK2A, TRICK2B, TRICKB, tumor necrosis factor receptor superfamily member 10B, KILLER/DR5 TRAIL death-inducing receptor, death receptor 5
  • Host
    Rabbit
  • Protein IDQ9QZM4
  • Protein Name
    Tumor necrosis factor receptor superfamily member 10B
  • Scientific Description
    The Death receptor 5 (DR5) Polyclonal Antibody (Species: Mouse) has been validated for the following applications: WB, IHC, ICC, IP.
  • Reactivity
    Mouse
  • Reactivity Supplier
    Mouse
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of DR5/Tnfrsf10b using anti-DR5/Tnfrsf10b antibody (A00410-4). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: mouse brain tissue lysates, Lane 3: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DR5/Tnfrsf10b antigen affinity purified polyclonal antibody (Catalog # A00410-4) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for DR5/Tnfrsf10b at approximately 48 kDa. The expected band size for DR5/Tnfrsf10b is at 48 kDa.
Product group Antibodies
Boster Bio
Anti-DR5/Tnfrsf10b Antibody Picoband(r)A00410-4-CARRIER-FREE
ApplicationsWestern Blot, ELISA
ReactivityMouse, Rat
TargetTnfrsf10b
  • SizePrice
Hela cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (DR5) polyclonal Antibody, Unconjugated (bs-1696R) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
Product group Antibodies
References
Bioss
DR5 Polyclonal AntibodyBS-1696R
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ReactivityHuman, Mouse, Rat
TargetTnfrsf10b
  • SizePrice
Product group Antibodies
GeneTex
DR5 antibody [9K26]GTX53422
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen
ReactivityMouse
TargetTnfrsf10b
  • SizePrice