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ProductsBack/DR5 antibody [9K26]

DR5 antibody [9K26]

GTX53422
GeneTex
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen
Product group Antibodies
ReactivityMouse
TargetTnfrsf10b
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Overview

  • Supplier
    GeneTex
  • Product Name
    DR5 antibody [9K26]
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:250 - 1:1000. IHC-Fr: 1:50 - 1:200. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    9K26
  • Concentration
    500 ug/ml
  • Conjugate
    Unconjugated
  • Gene ID21933
  • Target name
    Tnfrsf10b
  • Target description
    tumor necrosis factor receptor superfamily, member 10b
  • Target synonyms
    DR5, KILLER, Ly98, MK, TRAILR2, TRICK2A, TRICK2B, TRICKB, tumor necrosis factor receptor superfamily member 10B, KILLER/DR5 TRAIL death-inducing receptor, death receptor 5
  • Host
    Rat
  • Isotype
    IgG2
  • Protein IDQ9QZM4
  • Protein Name
    Tumor necrosis factor receptor superfamily member 10B
  • Reactivity
    Mouse
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of DR5/Tnfrsf10b using anti-DR5/Tnfrsf10b antibody (A00410-4). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: mouse brain tissue lysates, Lane 3: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DR5/Tnfrsf10b antigen affinity purified polyclonal antibody (Catalog # A00410-4) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for DR5/Tnfrsf10b at approximately 48 kDa. The expected band size for DR5/Tnfrsf10b is at 48 kDa.
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