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ProductsBack/DHFR Mouse Monoclonal Antibody [Clone ID: OTI3A11]
HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY DHFR (Cat# RC200089, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-DHFR antibody (Cat# TA500517). Positive lysates [LY400271] (100ug) and [LC400271] (20ug) can be purchased separately from OriGene.
HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY DHFR (Cat# RC200089, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-DHFR antibody (Cat# TA500517). Positive lysates [LY400271] (100ug) and [LC400271] (20ug) can be purchased separately from OriGene.
HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY DHFR (Cat# RC200089, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-DHFR antibody (Cat# TA500517). Positive lysates [LY400271] (100ug) and [LC400271] (20ug) can be purchased separately from OriGene.

DHFR Mouse Monoclonal Antibody [Clone ID: OTI3A11]

CF500517
OriGene
ApplicationsWestern Blot, ImmunoHistoChemistry
Product group Antibodies
TargetDHFR
100 ug
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Overview

  • Supplier
    OriGene
  • Product Name
    DHFR Mouse Monoclonal Antibody [Clone ID: OTI3A11]
  • Delivery Days Customer
    14
  • Applications
    Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    OTI3A11
  • Gene ID1719
  • Target name
    DHFR
  • Target description
    dihydrofolate reductase
  • Target synonyms
    DHFR1, DHFRP1, DYR, dihydrofolate reductase
  • Host
    Mouse
  • Isotype
    IgG2b
  • Protein IDP00374
  • Protein Name
    Dihydrofolate reductase
  • Scientific Description
    Carrier-free (BSA/glycerol-free) DHFR mouse monoclonal antibody, clone OTI3A11 (formerly 3A11)
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

MSDS

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Figure 1. Western blot analysis of DHFR using anti-DHFR antibody (PB9175). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: rat liver tissue lysates, Lane 4: rat kidney tissue lysates, Lane 5: mouse liver tissue lysates, Lane 6: mouse kidney tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DHFR antigen affinity purified polyclonal antibody (Catalog # PB9175) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for DHFR at approximately 21 kDa. The expected band size for DHFR is at 21 kDa.
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