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ProductsBack/DHFR Rabbit Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using DHFR antibody.
Western blot analysis of extracts of various cell lines, using DHFR antibody.
Western blot analysis of extracts of various cell lines, using DHFR antibody.

DHFR Rabbit Polyclonal Antibody

TA327096
OriGene
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry
Product group Antibodies
TargetDHFR
100 ul
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Overview

  • Supplier
    OriGene
  • Product Name
    DHFR Rabbit Polyclonal Antibody
  • Delivery Days Customer
    14
  • Applications
    ImmunoFluorescence, Western Blot, ImmunoCytoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Gene ID1719
  • Target name
    DHFR
  • Target description
    dihydrofolate reductase
  • Target synonyms
    DHFR1, DHFRP1, DYR, dihydrofolate reductase
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP00374
  • Protein Name
    Dihydrofolate reductase
  • Scientific Description
    Rabbit anti-DHFR Polyclonal Antibody
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

MSDS

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Figure 1. Western blot analysis of DHFR using anti-DHFR antibody (PB9175). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: rat liver tissue lysates, Lane 4: rat kidney tissue lysates, Lane 5: mouse liver tissue lysates, Lane 6: mouse kidney tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DHFR antigen affinity purified polyclonal antibody (Catalog # PB9175) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for DHFR at approximately 21 kDa. The expected band size for DHFR is at 21 kDa.
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