More than 130 suppliers
ISO certified
In-house tech support
we Connect you
Bio-Connect
ProductsBack/DHFR Rabbit Polyclonal Antibody
Gel: 12%SDS-PAGE
Lysate: 40 μg
Lane: 293T cell lysate
Primary antibody: TA366145 (DHFR Antibody) at dilution 1/400
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution
Exposure time: 1 minute
Gel: 12%SDS-PAGE<br>Lysate: 40 μg<br>Lane: 293T cell lysate<br>Primary antibody: TA366145 (DHFR Antibody) at dilution 1/400<br>Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution<br>Exposure time: 1 minute
Gel: 12%SDS-PAGE
Lysate: 40 μg
Lane: 293T cell lysate
Primary antibody: TA366145 (DHFR Antibody) at dilution 1/400
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution
Exposure time: 1 minute

DHFR Rabbit Polyclonal Antibody

TA366145
OriGene
ApplicationsWestern Blot, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetDHFR
Price on request
100 ul
Large volume orders?
Order with a bulk request
More than 130 suppliers
ISO certified
In-house tech support

Overview

  • Supplier
    OriGene
  • Product Name
    DHFR Rabbit Polyclonal Antibody
  • Delivery Days Customer
    14
  • Applications
    Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Gene ID1719
  • Target name
    DHFR
  • Target description
    dihydrofolate reductase
  • Target synonyms
    DHFR1, DHFRP1, DYR, dihydrofolate reductase
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP00374
  • Protein Name
    Dihydrofolate reductase
  • Scientific Description
    DHFR rabbit polyclonal antibody
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

MSDS

Download MSDS

FAQ

Go to our frequently asked questions

Related products

Product group Antibodies
RayBiotech
Anti-DHFR Antibody144-01607
ApplicationsImmunoFluorescence, Western Blot
ReactivityHuman, Mouse
TargetDHFR
  • SizePrice
Immunoprecipitation of DHFR protein from 293T whole cell extracts using 5 μg of DHFR antibody (GTX117705). Western blot analysis was performed using DHFR antibody (GTX117705). EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.
Product group Antibodies
References
GeneTex
DHFR antibodyGTX117705
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
ReactivityHuman, Rat
TargetDHFR
  • SizePrice
Product group Antibodies
Biomatik
Dhfr Polyclonal AntibodyCAC11829
ApplicationsImmunoFluorescence, ELISA, ImmunoHistoChemistry
TargetDHFR
  • SizePrice
Product group Antibodies
Bioss
DHFR Recombinant Antibody, AbBy Fluor-350 ConjugatedBSM-61547R-BF350
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry
ReactivityHuman, Mouse, Rat
TargetDHFR
  • SizePrice
Immunohistochemistry of paraffin-embedded human lung cancer using CSB-PA006847HA01HU at dilution of 1:100
Product group Antibodies
Cusabio
DHFR AntibodyCSB-PA006847HA01HU
ApplicationsImmunoFluorescence, ELISA, ImmunoHistoChemistry
ReactivityHuman
TargetDHFR
  • SizePrice
Anti-DHFR Antibody
Product group Antibodies
Antibodies.com
Anti-DHFR AntibodyA29840
ApplicationsWestern Blot, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
  • SizePrice
Product group Antibodies
LifeSpan BioSciences
DHFR AntibodyLS-C830690
ApplicationsELISA, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetDHFR
  • SizePrice
Product group Antibodies
MedChem Express
DHFR Antibody (YA3335, Rabbit)HY-P83590
TargetDHFR
  • SizePrice
Product group Antibodies
Assay Genie
SNRPD3 AntibodyCAB16070
ReactivityHuman
TargetDHFR
  • SizePrice
Figure 1. Western blot analysis of DHFR using anti-DHFR antibody (PB9175). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: rat liver tissue lysates, Lane 4: rat kidney tissue lysates, Lane 5: mouse liver tissue lysates, Lane 6: mouse kidney tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DHFR antigen affinity purified polyclonal antibody (Catalog # PB9175) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for DHFR at approximately 21 kDa. The expected band size for DHFR is at 21 kDa.
Product group Antibodies
Boster Bio
Anti-Dihydrofolate reductase (DHFR) Antibody Picoband(r)PB9175-CARRIER-FREE
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetDHFR
  • SizePrice