More than 130 suppliers
ISO certified
In-house tech support
we Connect you
Bio-Connect
ProductsBack/DHFR Recombinant Monoclonal Antibody

DHFR Recombinant Monoclonal Antibody

ORB689078
Biorbyt
ApplicationsImmunoPrecipitation, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetDHFR
Sign in to order and to see your custom pricing.
Large volume orders?
Order with a bulk request
More than 130 suppliers
ISO certified
In-house tech support

Overview

  • Supplier
    Biorbyt
  • Product Name
    DHFR Recombinant Monoclonal Antibody
  • Delivery Days Customer
    10
  • Applications
    ImmunoPrecipitation, ELISA, ImmunoHistoChemistry
  • Applications Supplier
    WB:1:500-1:5000, IHC-P:1:50-1:200, IP:1:200-1:1000 ELISA, IHC, IP, WB
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    9B2
  • Conjugate
    Unconjugated
  • Gene ID1719
  • Target name
    DHFR
  • Target description
    dihydrofolate reductase
  • Target synonyms
    DHFR1, DHFRP1, DYR, dihydrofolate reductase
  • Isotype
    IgG
  • Protein IDP00374
  • Protein Name
    Dihydrofolate reductase
  • Scientific Description
    Rabbit monoclonal antibody to DHFR
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

FAQ

Go to our frequently asked questions

Related products

Product group Antibodies
RayBiotech
Anti-DHFR Antibody144-01607
ApplicationsImmunoFluorescence, Western Blot
ReactivityHuman, Mouse
TargetDHFR
  • SizePrice
Immunoprecipitation of DHFR protein from 293T whole cell extracts using 5 μg of DHFR antibody (GTX117705). Western blot analysis was performed using DHFR antibody (GTX117705). EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.
Product group Antibodies
References
GeneTex
DHFR antibodyGTX117705
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
ReactivityHuman, Rat
TargetDHFR
  • SizePrice
Product group Antibodies
Biomatik
Dhfr Polyclonal AntibodyCAC11829
ApplicationsImmunoFluorescence, ELISA, ImmunoHistoChemistry
TargetDHFR
  • SizePrice
Product group Antibodies
Bioss
DHFR Recombinant Antibody, AbBy Fluor-350 ConjugatedBSM-61547R-BF350
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry
ReactivityHuman, Mouse, Rat
TargetDHFR
  • SizePrice
Immunohistochemistry of paraffin-embedded human lung cancer using CSB-PA006847HA01HU at dilution of 1:100
Product group Antibodies
Cusabio
DHFR AntibodyCSB-PA006847HA01HU
ApplicationsImmunoFluorescence, ELISA, ImmunoHistoChemistry
ReactivityHuman
TargetDHFR
  • SizePrice
Anti-DHFR Antibody
Product group Antibodies
Antibodies.com
Anti-DHFR AntibodyA29840
ApplicationsWestern Blot, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
  • SizePrice
Product group Antibodies
LifeSpan BioSciences
DHFR AntibodyLS-C830690
ApplicationsELISA, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetDHFR
  • SizePrice
Product group Antibodies
MedChem Express
DHFR Antibody (YA3335, Rabbit)HY-P83590
TargetDHFR
  • SizePrice
Product group Antibodies
Assay Genie
SNRPD3 AntibodyCAB16070
ReactivityHuman
TargetDHFR
  • SizePrice
Figure 1. Western blot analysis of DHFR using anti-DHFR antibody (PB9175). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: rat liver tissue lysates, Lane 4: rat kidney tissue lysates, Lane 5: mouse liver tissue lysates, Lane 6: mouse kidney tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DHFR antigen affinity purified polyclonal antibody (Catalog # PB9175) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for DHFR at approximately 21 kDa. The expected band size for DHFR is at 21 kDa.
Product group Antibodies
Boster Bio
Anti-Dihydrofolate reductase (DHFR) Antibody Picoband(r)PB9175-CARRIER-FREE
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetDHFR
  • SizePrice