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ProductsBack/DHPS Mouse Monoclonal Antibody [Clone ID: OTI2C9]
HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY DHPS (Cat# RC204235, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-DHPS(Cat# TA501393). Positive lysates [LY400714] (100ug) and [LC400714] (20ug) can be purchased separately from OriGene.
HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY DHPS (Cat# RC204235, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-DHPS(Cat# TA501393). Positive lysates [LY400714] (100ug) and [LC400714] (20ug) can be purchased separately from OriGene.
HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY DHPS (Cat# RC204235, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-DHPS(Cat# TA501393). Positive lysates [LY400714] (100ug) and [LC400714] (20ug) can be purchased separately from OriGene.

DHPS Mouse Monoclonal Antibody [Clone ID: OTI2C9]

CF501393
OriGene
ApplicationsWestern Blot
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetDHPS
Price on request
100 ug
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Overview

  • Supplier
    OriGene
  • Product Name
    DHPS Mouse Monoclonal Antibody [Clone ID: OTI2C9]
  • Delivery Days Customer
    14
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    OTI2C9
  • Gene ID1725
  • Target name
    DHPS
  • Target description
    deoxyhypusine synthase
  • Target synonyms
    DHS, DS, MIG13, NEDSSWI, deoxyhypusine synthase, migration-inducing gene 13
  • Host
    Mouse
  • Isotype
    IgG2a
  • Protein IDP49366
  • Protein Name
    Deoxyhypusine synthase
  • Scientific Description
    Carrier-free (BSA/glycerol-free) DHPS mouse monoclonal antibody, clone OTI2C9 (formerly 2C9)
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

MSDS

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Figure 1. Western blot analysis of DHPS/DHS using anti-DHPS/DHS antibody (A02106-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human MCF-7 whole cell lysates, Lane 3: human Jurkat whole cell lysates, Lane 4: human HEK293 whole cell lysates, Lane 5: human SH-SY5Y whole cell lysates, Lane 6: human THP-1 whole cell lysates, Lane 7: human PC-3 whole cell lysates, Lane 8: human U937 whole cell lysates, Lane 9: rat testis tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DHPS/DHS antigen affinity purified polyclonal antibody (Catalog # A02106-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for DHPS/DHS at approximately 41 kDa. The expected band size for DHPS/DHS is at 41 kDa.
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