Western blot using GeneTex Affinity Purified anti-DIAPH2 antibody (GTX10562) shows detection of a 132-kDa band corresponding to DIAPH2 in a lysate prepared from human derived HEK293 cells. Approximately 20 ug of lysate was run on a SDS-PAGE and transferred onto nitrocellulose followed by reaction with a 1:500 dilution of anti-DIA-2 antibody. Detection occurred using a 1:5,000 dilution of HRP-labeled Goat anti-Rabbit IgG for 1 hour at room temperature. A chemiluminescence system was used for signal detection (Roche) using a 1 min exposure time.
Western blot using GeneTex Affinity Purified anti-DIAPH2 antibody (GTX10562) shows detection of a 132-kDa band corresponding to DIAPH2 in a lysate prepared from human derived HEK293 cells. Approximately 20 ug of lysate was run on a SDS-PAGE and transferred onto nitrocellulose followed by reaction with a 1:500 dilution of anti-DIA-2 antibody. Detection occurred using a 1:5,000 dilution of HRP-labeled Goat anti-Rabbit IgG for 1 hour at room temperature. A chemiluminescence system was used for signal detection (Roche) using a 1 min exposure time.
DIAPH2 antibody
GTX10562
ApplicationsImmunoPrecipitation, Western Blot, ELISA
Product group Antibodies
TargetDIAPH2
Overview
- SupplierGeneTex
- Product NameDIAPH2 antibody
- Delivery Days Customer9
- Application Supplier NoteWB: 1:1000-1:6000. IP: 1:100. ELISA: 1:12000-1:60000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsImmunoPrecipitation, Western Blot, ELISA
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration1.4 mg/ml
- ConjugateUnconjugated
- Gene ID1730
- Target nameDIAPH2
- Target descriptiondiaphanous related formin 2
- Target synonymsDIA, DIA2, DRF2, POF, POF2, POF2A, protein diaphanous homolog 2, Premature ovarian failure 2A, diaphanous homolog 2, diaphorase-2
- HostRabbit
- IsotypeIgG
- Protein IDO60879
- Protein NameProtein diaphanous homolog 2
- Scientific DescriptionThis gene may play a role in the development and normal function of the ovaries. Mutations of this gene have been linked to premature ovarian failure. Alternative splicing results in two protein isoforms. Furthermore, each splice variant undergoes additional splicing in the 3 UTR. Overall 4 splice variants have been described.
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203
Datasheet
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