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Formalin-fixed and paraffin embedded rat kidney labeled with Rabbit Anti-DIAPH2/Dia2 Polyclonal Antibody, Unconjugated (bs-13002R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded rat kidney labeled with Rabbit Anti-DIAPH2/Dia2 Polyclonal Antibody, Unconjugated (bs-13002R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded rat kidney labeled with Rabbit Anti-DIAPH2/Dia2 Polyclonal Antibody, Unconjugated (bs-13002R) at 1:200 followed by conjugation to the secondary antibody and DAB staining

DIAPH2 Polyclonal Antibody

BS-13002R
Bioss Antibodies
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
DownloadDatasheet
Product group Antibodies
TargetDIAPH2
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Overview

  • Supplier
    Bioss Antibodies
  • Product Name
    DIAPH2/Dia2 Polyclonal Antibody
  • Delivery Days Customer
    16
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    WB(1:300-5000), ELISA(1:500-1000), IHC-P(1:200-400), IHC-F(1:100-500), IF(IHC-P)(1:50-200), IF(IHC-F)(1:50-200), IF(ICC)(1:50-200)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 ug/ul
  • Conjugate
    Unconjugated
  • Gene ID1730
  • Target name
    DIAPH2
  • Target description
    diaphanous related formin 2
  • Target synonyms
    DIA; DIA2; diaphanous homolog 2; diaphorase-2; DRF2; POF; POF2; POF2A; protein diaphanous homolog 2
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO60879
  • Protein Name
    Protein diaphanous homolog 2
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Western blot using GeneTex Affinity Purified anti-DIAPH2 antibody (GTX10562) shows detection of a 132-kDa band corresponding to DIAPH2 in a lysate prepared from human derived HEK293 cells. Approximately 20 ug of lysate was run on a SDS-PAGE and transferred onto nitrocellulose followed by reaction with a 1:500 dilution of anti-DIA-2 antibody. Detection occurred using a 1:5,000 dilution of HRP-labeled Goat anti-Rabbit IgG for 1 hour at room temperature. A chemiluminescence system was used for signal detection (Roche) using a 1 min exposure time.
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Western blot All lanes: DIAPH2 antibody at 4.75ug/ml Lane 1: 293T whole cell lysate Lane 2: Hela whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 126, 125 kDa Observed band size: 126 kDa
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