Paraformaldehyde-fixed, paraffin embedded Rat liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with DIAPH3 Polyclonal Antibody, Unconjugated (bs-14325R) at 1:200 overnight at 4°C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Rat liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with DIAPH3 Polyclonal Antibody, Unconjugated (bs-14325R) at 1:200 overnight at 4°C, DAB staining.
DIAPH3 Polyclonal Antibody
BS-14325R
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetDIAPH3
Overview
- SupplierBioss Antibodies
- Product NameDIAPH3 Polyclonal Antibody
- Delivery Days Customer16
- ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
- Applications SupplierWB(1:300-5000), ELISA(1:500-1000), IHC-P(1:200-400), IHC-F(1:100-500), IF(IHC-P)(1:50-200), IF(IHC-F)(1:50-200), IF(ICC)(1:50-200)
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration1 ug/ul
- ConjugateUnconjugated
- Gene ID81624
- Target nameDIAPH3
- Target descriptiondiaphanous related formin 3
- Target synonymsAN; AUNA1; DIA2; diap3; diaphanous homolog 3; DRF3; mDia2; NSDAN; protein diaphanous homolog 3
- HostRabbit
- IsotypeIgG
- Protein IDQ9NSV4
- Protein NameProtein diaphanous homolog 3
- Storage Instruction-20°C
- UNSPSC12352203
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