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DIO1 Antibody

CSB-PA343445LA01HU
Cusabio
ApplicationsELISA
Product group Antibodies
ReactivityHuman
TargetDIO1
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Overview

  • Supplier
    Cusabio
  • Product Name
    DIO1 Antibody
  • Delivery Days Customer
    20
  • Applications
    ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID1733
  • Target name
    DIO1
  • Target description
    iodothyronine deiodinase 1
  • Target synonyms
    5DI, THMA2, TXDI1, type I iodothyronine deiodinase, DIOI, deiodinase, iodothyronine type I, iodothyronine deiodinase type 1, selenoprotein DIO1, thyroxine 5'-deiodinase, thyroxine deiodinase type I (selenoprotein), type 1 DI, type-I 5'-deiodinase
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP49895
  • Protein Name
    Type I iodothyronine deiodinase
  • Scientific Description
    Responsible for the deiodination of T4 (3,5,3,5-tetraiodothyronine) into T3 (3,5,3-triiodothyronine) and of T3 into T2 (3,3-diiodothyronine). Plays a role in providing a source of plasma T3 by deiodination of T4 in peripheral tissues such as liver and kidney.
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of DIO1 using anti-DIO1 antibody (A04612). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat liver tissue lysates, Lane 2: rat kidney tissue lysates, Lane 3: human A549 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DIO1 antigen affinity purified polyclonal antibody (Catalog # A04612) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for DIO1 at approximately 29KD. The expected band size for DIO1 is at 29KD.
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