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DIO1 Antibody

PACO34530
Assay Genie
ApplicationsELISA
Product group Antibodies
ReactivityHuman
TargetDIO1
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Overview

  • Supplier
    Assay Genie
  • Product Name
    DIO1 Antibody
  • Delivery Days Customer
    9
  • Applications
    ELISA
  • Applications Supplier
    ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Clone ID
    Not applicable
  • Conjugate
    Unconjugated
  • Gene ID1733
  • Target name
    DIO1
  • Target description
    iodothyronine deiodinase 1
  • Target synonyms
    5DI, THMA2, TXDI1, type I iodothyronine deiodinase, DIOI, deiodinase, iodothyronine type I, iodothyronine deiodinase type 1, selenoprotein DIO1, thyroxine 5'-deiodinase, thyroxine deiodinase type I (selenoprotein), type 1 DI, type-I 5'-deiodinase
  • Host
    Rabbit
  • Protein IDP49895
  • Protein Name
    Type I iodothyronine deiodinase
  • Scientific Description
    DIO1 Antibody is a premium polyclonal that offers outstanding performance and reliability for demanding research applications. Rigorously validated for ELISA, this antibody ensures consistent, reproducible results across multiple experimental platforms. Demonstrates excellent reactivity with Human samples, providing researchers with confidence in cross-species compatibility. Conveniently packaged in 50ug format to meet your experimental needs. For optimal performance, store at -20°C or -80°C and maintains stability for 12 months. Backed by rigorous quality control testing to ensure superior performance in your critical research applications.
  • Shelf life instruction
    12 months
  • Source
    Rabbit
  • Reactivity
    Human
  • Reactivity Supplier
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

Datasheet

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MSDS

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Figure 1. Western blot analysis of DIO1 using anti-DIO1 antibody (A04612). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat liver tissue lysates, Lane 2: rat kidney tissue lysates, Lane 3: human A549 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DIO1 antigen affinity purified polyclonal antibody (Catalog # A04612) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for DIO1 at approximately 29KD. The expected band size for DIO1 is at 29KD.
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