
WB analysis of MCF-7 cell lysate (35ug/lane) using GTX81072 DIO2 antibody, Internal.
DIO2 antibody, Internal
GTX81072
Overview
- SupplierGeneTex
- Product NameDIO2 antibody, Internal
- Delivery Days Customer9
- Application Supplier NoteWB: 1:1000. IHC-P: 1:50-1:100. FACS: 1:10-1:50. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- FormulationLiquid
- Gene ID1734
- Target nameDIO2
- Target descriptioniodothyronine deiodinase 2
- Target synonyms5DII; D2; deiodinase, iodothyronine type II; deiodinase-2; deiodonase-2; DIOII; selenoprotein Y; SELENOY; SelY; thyroxine deiodinase, type II; TXDI2; type 2 DI; type 2 iodothyronine deiodinase; type II iodothyronine deiodinase; type-II 5'deiodinase; type-II 5'-deiodinase
- Protein IDQ92813
- Protein NameType II iodothyronine deiodinase
- Scientific DescriptionThe protein encoded by this gene belongs to the iodothyronine deiodinase family. It catalyzes the conversion of prohormone thyroxine (3,5,3,5-tetraiodothyronine, T4) to the bioactive thyroid hormone (3,5,3-triiodothyronine, T3) by outer ring 5-deiodination. This gene is widely expressed, including in thyroid and brain. It is thought to be responsible for the local production of T3, and thus important in influencing thyroid hormone action in these tissues. It has also been reported to be highly expressed in thyroids of patients with Graves disease, and in follicular adenomas. The intrathyroidal T4 to T3 conversion by this enzyme may contribute significantly to the relative increase in thyroidal T3 production in these patients. This protein is a selenoprotein containing the non-standard amino acid, selenocysteine (Sec), which is encoded by the UGA codon that normally signals translation termination. The 3 UTRs of selenoprotein mRNAs contain a conserved stem-loop structure, designated the Sec insertion sequence (SECIS) element, that is necessary for the recognition of UGA as a Sec codon, rather than as a stop signal. Unlike the other two members (DIO1 and DIO3) of this enzyme family, the mRNA for this gene contains an additional in-frame UGA codon that has been reported (in human) to function either as a Sec or a stop codon, which can result in two isoforms with one or two Sec residues; however, only the upstream Sec (conserved with the single Sec residue found at the active site in DIO1 and DIO3) was shown to be essential for enzyme activity (PMID:10403186). Alternatively spliced transcript variants have been described for this gene. [provided by RefSeq, Oct 2018]
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203