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ProductsBack/DKK1 Mouse Monoclonal Antibody [Clone ID: 2H2]
Western blot analysis using DKK1 antibody Cat.-No AM06319SU-N against HEK293 (Lane 1) and DKK1-hIgGFc transfected HEK293 cell lysate (Lane 2).
Western blot analysis using DKK1 antibody Cat.-No AM06319SU-N against HEK293 (Lane 1) and DKK1-hIgGFc transfected HEK293 cell lysate (Lane 2).
Western blot analysis using DKK1 antibody Cat.-No AM06319SU-N against HEK293 (Lane 1) and DKK1-hIgGFc transfected HEK293 cell lysate (Lane 2).

DKK1 Mouse Monoclonal Antibody [Clone ID: 2H2]

AM06319SU-N
OriGene
ApplicationsWestern Blot
Product group Antibodies
ReactivityHuman
TargetDKK1
Price on request
100 ul
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Overview

  • Supplier
    OriGene
  • Product Name
    DKK1 Mouse Monoclonal Antibody [Clone ID: 2H2]
  • Delivery Days Customer
    14
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    2H2
  • Gene ID22943
  • Target name
    DKK1
  • Target description
    dickkopf Wnt signaling pathway inhibitor 1
  • Target synonyms
    DKK-1, SK, dickkopf-related protein 1, dickkopf 1 homolog, dickkopf-1 like, dickkopf-like protein 1
  • Host
    Mouse
  • Isotype
    IgG2b
  • Protein IDO94907
  • Protein Name
    Dickkopf-related protein 1
  • Scientific Description
    DKK1 mouse monoclonal antibody, clone 2H2, Ascites
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

MSDS

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Figure 1. Western blot analysis of DKK1 using anti-DKK1 antibody (A00632). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: recombinant human DKK1 protein 1ng. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DKK1 antigen affinity purified polyclonal antibody (Catalog # A00632) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for DKK1 at approximately 38KD. The expected band size for DKK1 is at 26KD.
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