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Western Blot Positive WB detected in: HepG2 whole cell lysate All lanes: DLAT antibody at 4ug/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 69 kDa Observed band size: 69 kDa
Western Blot Positive WB detected in: HepG2 whole cell lysate All lanes: DLAT antibody at 4ug/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 69 kDa Observed band size: 69 kDa
Western Blot Positive WB detected in: HepG2 whole cell lysate All lanes: DLAT antibody at 4ug/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 69 kDa Observed band size: 69 kDa

DLAT Antibody

CSB-PA006926LA01HU
Cusabio
ApplicationsImmunoPrecipitation, Western Blot, ELISA
Product group Antibodies
ReactivityHuman
TargetDLAT
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Overview

  • Supplier
    Cusabio
  • Product Name
    DLAT Antibody
  • Delivery Days Customer
    20
  • Applications
    ImmunoPrecipitation, Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID1737
  • Target name
    DLAT
  • Target description
    dihydrolipoamide S-acetyltransferase
  • Target synonyms
    DLTA, E2, PBC, PDC-E2, PDCE2, dihydrolipoyllysine-residue acetyltransferase component of pyruvate dehydrogenase complex, mitochondrial, 70 kDa mitochondrial autoantigen of primary biliary cirrhosis, E2 component of pyruvate dehydrogenase complex, M2 antigen complex 70 kDa subunit, dihydrolipoamide acetyltransferase component of pyruvate dehydrogenase complex, pyruvate dehydrogenase complex component E2
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP10515
  • Protein Name
    Dihydrolipoyllysine-residue acetyltransferase component of pyruvate dehydrogenase complex, mitochondrial
  • Scientific Description
    The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2), and thereby links the glycolytic pathway to the tricarboxylic cycle.
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of DLAT using anti-DLAT antibody (A04469-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human HEPG2 whole cell lysates, Lane 3: human K562 whole cell lysates, Lane 4: human T-47D whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DLAT antigen affinity purified polyclonal antibody (Catalog # A04469-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for DLAT at approximately 69 kDa. The expected band size for DLAT is at 69 kDa.
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