More than 130 suppliers
ISO certified
In-house tech support
we Connect you
Bio-Connect
ProductsBack/DLAT antibody

DLAT antibody

GTX109766
GeneTex
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
DownloadDatasheet
Product group Antibodies
TargetDLAT
Sign in to order and to see your custom pricing.
Large volume orders?
Order with a bulk request
More than 130 suppliers
ISO certified
In-house tech support

Overview

  • Supplier
    GeneTex
  • Product Name
    DLAT antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:5000-1:20000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID1737
  • Target name
    DLAT
  • Target description
    dihydrolipoamide S-acetyltransferase
  • Target synonyms
    70 kDa mitochondrial autoantigen of primary biliary cirrhosis; dihydrolipoamide acetyltransferase component of pyruvate dehydrogenase complex; dihydrolipoyllysine-residue acetyltransferase component of pyruvate dehydrogenase complex, mitochondrial; DLTA; E2; E2 component of pyruvate dehydrogenase complex; M2 antigen complex 70 kDa subunit; PBC; PDCE2; PDC-E2; pyruvate dehydrogenase complex component E2
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP10515
  • Protein Name
    Dihydrolipoyllysine-residue acetyltransferase component of pyruvate dehydrogenase complex, mitochondrial
  • Scientific Description
    The DLAT gene encodes dihydrolipoamide acetyltransferase (EC 2.3.1.12), the E2 subunit of the mammalian pyruvate dehydrogenase complex (PDC; EC 1.2.4.1) of the inner mitochondrial membrane. Patients with primary biliary cirrhosis (PBC; MIM 109720) show autoantibodies to DLAT.[supplied by OMIM]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

References

  • Glutathionylation of pyruvate dehydrogenase complex E2 and inflammatory cytokine production during acute inflammation are magnified by mitochondrial oxidative stress.
    Read more
  • Glutathionylation of Pyruvate Dehydrogenase Complex E2 and Inflammatory Cytokine Production During Acute Inflammation Are Magnified By Mitochondrial Oxidative Stress.
    Read more
  • Focal adhesion ribonucleoprotein complex proteins are major humoral cancer antigens and targets in autoimmune diseases. Atsumi S et al., 2020 Oct 16, Commun Biol
    Read more

Related products

Product group Antibodies
RayBiotech
Anti-DLAT Antibody144-60697
ApplicationsWestern Blot, ImmunoHistoChemistry
TargetDLAT
  • SizePrice
Product group Antibodies
Biomatik
DLAT Polyclonal AntibodyCAC12898
ApplicationsImmunoPrecipitation, Western Blot, ELISA
TargetDLAT
  • SizePrice
Product group Antibodies
Assay Genie
DLAT AntibodyCAB6288
TargetDLAT
  • SizePrice
Product group Antibodies
Bioss Antibodies
DLAT Recombinant Antibody, AbBy Fluor-405 ConjugatedBSM-62155R-BF405
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry
TargetDLAT
  • SizePrice
Product group Antibodies
MedChem Express
Pyruvate Dehydrogenase E2 Antibody (YA113, Rabbit)HY-P80292
TargetDLAT
  • SizePrice
Western Blot Positive WB detected in: HepG2 whole cell lysate All lanes: DLAT antibody at 4ug/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 69 kDa Observed band size: 69 kDa
Product group Antibodies
Cusabio
DLAT AntibodyCSB-PA006926LA01HU
ApplicationsImmunoPrecipitation, Western Blot, ELISA
ReactivityHuman
TargetDLAT
  • SizePrice
Immunohistochemistry analysis in human heart muscle and liver tissues using Anti-DLAT antibody. Corresponding DLAT RNA-seq data are presented for the same tissues.
Product group Antibodies
Atlas Antibodies
Anti-DLAT AntibodyHPA040786
ApplicationsWestern Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHuman
TargetDLAT
  • SizePrice
Figure 1. Western blot analysis of DLAT using anti-DLAT antibody (A04469-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human HEPG2 whole cell lysates, Lane 3: human K562 whole cell lysates, Lane 4: human T-47D whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DLAT antigen affinity purified polyclonal antibody (Catalog # A04469-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for DLAT at approximately 69 kDa. The expected band size for DLAT is at 69 kDa.
Product group Antibodies
Boster Bio
Anti-DLAT Antibody Picoband(r)A04469-2-CARRIER-FREE
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry
TargetDLAT
  • SizePrice
WB analysis of various sample lysates using GTX54002 DLAT antibody. Dilution : 1:1000 Loading : 25microg per lane
Product group Antibodies
GeneTex
DLAT antibodyGTX54002
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry
TargetDLAT
  • SizePrice
WB analysis of HepG2 cells using GTX47092 DLAT antibody at 0.2-1microg/ml.
Product group Antibodies
GeneTex
DLAT antibody, C-termGTX47092
ApplicationsWestern Blot
TargetDLAT
  • SizePrice