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Western Blot Positive WB detected in: A549 whole cell lysate, MCF-7 whole cell lysate, HepG2 whole cell lysate, SH-SY5Y whole cell lysate, Rat brain tissue All lanes: DLK1 antibody at 4.6microg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 42, 34 kDa Observed band size: 42 kDa
Western Blot Positive WB detected in: A549 whole cell lysate, MCF-7 whole cell lysate, HepG2 whole cell lysate, SH-SY5Y whole cell lysate, Rat brain tissue All lanes: DLK1 antibody at 4.6microg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 42, 34 kDa Observed band size: 42 kDa
Western Blot Positive WB detected in: A549 whole cell lysate, MCF-7 whole cell lysate, HepG2 whole cell lysate, SH-SY5Y whole cell lysate, Rat brain tissue All lanes: DLK1 antibody at 4.6microg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 42, 34 kDa Observed band size: 42 kDa

DLK1 Antibody

CSB-PA006945LA01HU
Cusabio
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Rat
TargetDLK1
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Overview

  • Supplier
    Cusabio
  • Product Name
    DLK1 Antibody
  • Delivery Days Customer
    20
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID8788
  • Target name
    DLK1
  • Target description
    delta like non-canonical Notch ligand 1
  • Target synonyms
    Delta1; delta-like 1 homolog; DLK; DLK-1; FA1; fetal antigen 1; pG2; preadipocyte factor 1; PREF1; Pref-1; protein delta homolog 1; secredeltin; ZOG
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP80370
  • Protein Name
    Protein delta homolog 1
  • Scientific Description
    May have a role in neuroendocrine differentiation.
  • Reactivity
    Human, Rat
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of DLK-1/DLK1 using anti-DLK-1/DLK1 antibody (A00513-5). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human placenta tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DLK-1/DLK1 antigen affinity purified polyclonal antibody (Catalog # A00513-5) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for DLK-1/DLK1 at approximately 50 kDa. The expected band size for DLK-1/DLK1 is at 50 kDa.
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