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DLK1 Antibody (YA4139, Mouse)

HY-P84442
MedChem Express
TargetDLK1
Product group Antibodies
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Overview

  • Supplier
    MedChem Express
  • Product Name
    DLK1 Antibody (YA4139, Mouse)
  • Delivery Days Customer
    5
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Gene ID8788
  • Target name
    DLK1
  • Target description
    delta like non-canonical Notch ligand 1
  • Target synonyms
    DLK, DLK-1, Delta1, FA1, PREF1, Pref-1, ZOG, pG2, protein delta homolog 1, delta-like 1 homolog, fetal antigen 1, preadipocyte factor 1, secredeltin
  • Host
    Mouse
  • Isotype
    IgG1
  • Scientific Description
    DLK1 Antibody (YA4139) is a mouse-derived and non-conjugated IgG1 antibody, targeting to DLK1. It can be applicated for ICC/IF, FC, ELISA assays, in the background of human.
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of DLK-1/DLK1 using anti-DLK-1/DLK1 antibody (A00513-5). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human placenta tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DLK-1/DLK1 antigen affinity purified polyclonal antibody (Catalog # A00513-5) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for DLK-1/DLK1 at approximately 50 kDa. The expected band size for DLK-1/DLK1 is at 50 kDa.
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