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ProductsBack/DNA Polymerase epsilon p59 antibody
The data was published in the journal PLoS One in 2016.
The data was published in the journal PLoS One in 2016.
The data was published in the journal PLoS One in 2016.

DNA Polymerase epsilon p59 antibody

GTX109069
GeneTex
ApplicationsDot Blot
Product group Antibodies
ReactivityHuman
TargetPOLE2
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Overview

  • Supplier
    GeneTex
  • Product Name
    DNA Polymerase epsilon p59 antibody
  • Delivery Days Customer
    9
  • Applications
    Dot Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID5427
  • Target name
    POLE2
  • Target description
    DNA polymerase epsilon 2, accessory subunit
  • Target synonyms
    DPE2, DNA polymerase epsilon subunit 2, DNA polymerase II subunit 2, DNA polymerase epsilon subunit B, polymerase (DNA directed), epsilon 2 (p59 subunit), polymerase (DNA directed), epsilon 2, accessory subunit, polymerase (DNA) epsilon 2, accessory subunit
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP56282
  • Protein Name
    DNA polymerase epsilon subunit 2
  • Scientific Description
    Participates in DNA repair and in chromosomal DNA replication.
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of POLE2 using anti-POLE2 antibody (A09182-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human PC-3 whole cell lysates, Lane 2: human U20S whole cell lysates, Lane 3: human A431 whole cell lysates, Lane 4: human Jurkat whole cell lysates, Lane 5: rat RH35 whole cell lysates, Lane 6: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-POLE2 antigen affinity purified polyclonal antibody (Catalog # A09182-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for POLE2 at approximately 65 kDa. The expected band size for POLE2 is at 60 kDa.
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