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POLE2 Antibody

LS-C747914
LifeSpan BioSciences
ApplicationsWestern Blot
Product group Antibodies
TargetPOLE2
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    POLE2 Antibody
  • Delivery Days Customer
    23
  • Application Supplier Note
    The predicted MW is 56kDa/59kDa, while the observed MW by Western blot was 59kDa.
  • Applications
    Western Blot
  • Applications Supplier
    WB (1:500 - 1:2000) The predicted MW is 56kDa/59kDa, while the observed MW by Western blot was 59kDa.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID5427
  • Target name
    POLE2
  • Target description
    DNA polymerase epsilon 2, accessory subunit
  • Target synonyms
    DPE2, DNA polymerase epsilon subunit 2, DNA polymerase II subunit 2, DNA polymerase epsilon subunit B, polymerase (DNA directed), epsilon 2 (p59 subunit), polymerase (DNA directed), epsilon 2, accessory subunit, polymerase (DNA) epsilon 2, accessory subunit
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of POLE2 using anti-POLE2 antibody (A09182-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human PC-3 whole cell lysates, Lane 2: human U20S whole cell lysates, Lane 3: human A431 whole cell lysates, Lane 4: human Jurkat whole cell lysates, Lane 5: rat RH35 whole cell lysates, Lane 6: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-POLE2 antigen affinity purified polyclonal antibody (Catalog # A09182-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for POLE2 at approximately 65 kDa. The expected band size for POLE2 is at 60 kDa.
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