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DNAJA2 Antibody

LS-C349019
LifeSpan BioSciences
ApplicationsWestern Blot, ImmunoHistoChemistry
Product group Antibodies
TargetDNAJA2
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    DNAJA2 Antibody
  • Delivery Days Customer
    23
  • Application Supplier Note
    The predicted MW is 45kDa, while the observed MW by Western blot was 46kDa.
  • Applications
    Western Blot, ImmunoHistoChemistry
  • Applications Supplier
    IHC (1:50 - 1:200), WB (1:500 - 1:2000) The predicted MW is 45kDa, while the observed MW by Western blot was 46kDa.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID10294
  • Target name
    DNAJA2
  • Target description
    DnaJ heat shock protein family (Hsp40) member A2
  • Target synonyms
    cell cycle progression 3 protein; cell cycle progression restoration gene 3 protein; CPR3; DJ3; DJA2; DNAJ; DnaJ (Hsp40) homolog, subfamily A, member 2; dnaJ homolog subfamily A member 2; DNJ3; HIRA interacting protein 4; HIRIP4; PRO3015; RDJ2; renal carcinoma antigen NY-REN-14
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of DNAJA2 using anti-DNAJA2 antibody (A08918-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human K562 whole cell lysates, Lane 4: human Raji whole cell lysates, Lane 5: rat kidney tissue lysates, Lane 6: mouse testis tissue lysates, Lane 7: mouse NIH-3T3 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DNAJA2 antigen affinity purified polyclonal antibody (Catalog # A08918-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for DNAJA2 at approximately 46KD. The expected band size for DNAJA2 is at 46KD.
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