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Immunofluorescent staining of human cell line MCF7 shows localization to nucleoli & cytosol.
Immunofluorescent staining of human cell line MCF7 shows localization to nucleoli & cytosol.
Immunofluorescent staining of human cell line MCF7 shows localization to nucleoli & cytosol.

Anti-DNAJA2 Antibody

HPA049789
Atlas Antibodies
ApplicationsWestern Blot, ImmunoCytoChemistry
Product group Antibodies
ReactivityHuman
TargetDNAJA2
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-DNAJA2 Antibody
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoCytoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID10294
  • Target name
    DNAJA2
  • Target description
    DnaJ heat shock protein family (Hsp40) member A2
  • Target synonyms
    CPR3, DJ3, DJA2, DNAJ, DNJ3, HIRIP4, PRO3015, RDJ2, dnaJ homolog subfamily A member 2, DnaJ (Hsp40) homolog, subfamily A, member 2, HIRA interacting protein 4, cell cycle progression 3 protein, cell cycle progression restoration gene 3 protein, renal carcinoma antigen NY-REN-14
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO60884
  • Protein Name
    DnaJ homolog subfamily A member 2
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of DNAJA2 using anti-DNAJA2 antibody (A08918-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human K562 whole cell lysates, Lane 4: human Raji whole cell lysates, Lane 5: rat kidney tissue lysates, Lane 6: mouse testis tissue lysates, Lane 7: mouse NIH-3T3 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DNAJA2 antigen affinity purified polyclonal antibody (Catalog # A08918-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for DNAJA2 at approximately 46KD. The expected band size for DNAJA2 is at 46KD.
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