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The image on the left is immunohistochemistry of paraffin-embedded Human ovarian cancer tissue using CSB-PA048817(DNAJC10 Antibody) at dilution 1/20, on the right is treated with fusion protein. (Original magnification: x200)
The image on the left is immunohistochemistry of paraffin-embedded Human ovarian cancer tissue using CSB-PA048817(DNAJC10 Antibody) at dilution 1/20, on the right is treated with fusion protein. (Original magnification: x200)
The image on the left is immunohistochemistry of paraffin-embedded Human ovarian cancer tissue using CSB-PA048817(DNAJC10 Antibody) at dilution 1/20, on the right is treated with fusion protein. (Original magnification: x200)

DNAJC10 Antibody

CSB-PA048817
Cusabio
ApplicationsELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetDNAJC10
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Overview

  • Supplier
    Cusabio
  • Product Name
    DNAJC10 Antibody
  • Delivery Days Customer
    20
  • Applications
    ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID54431
  • Target name
    DNAJC10
  • Target description
    DnaJ heat shock protein family (Hsp40) member C10
  • Target synonyms
    ERdj5, JPDI, MTHr, PDIA19, dnaJ homolog subfamily C member 10, DnaJ (Hsp40) homolog, subfamily C, member 10, ER-resident protein ERdj5, J-domain-containing protein disulfide isomerase-like protein, endoplasmic reticulum DNA J domain-containing protein 5, macrothioredoxin, protein disulfide isomerase family A, member 19
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ8IXB1
  • Protein Name
    DnaJ homolog subfamily C member 10
  • Scientific Description
    This gene encodes an endoplasmic reticulum co-chaperone which is part of the endoplasmic reticulum-associated degradation complex involved in recognizing and degrading misfolded proteins. The encoded protein reduces incorrect disulfide bonds in misfolded glycoproteins. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of DNAJC10 using anti-DNAJC10 antibody (A07954-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human HELA whole cell lysates, Lane 2: human HEPG2 whole cell lysates, Lane 3: human U87 whole cell lysates, Lane 4: rat stomach tissue lysates, Lane 5: rat RH35 whole cell lysates, Lane 6: mouse stomach tissue lysates, Lane 7: mouse HEPA1-6 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DNAJC10 antigen affinity purified polyclonal antibody (Catalog # A07954-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for DNAJC10 at approximately 91KD. The expected band size for DNAJC10 is at 91KD.
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