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DNAJC10 Antibody

LS-C747322
LifeSpan BioSciences
ApplicationsWestern Blot
Product group Antibodies
TargetDNAJC10
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    DNAJC10 Antibody
  • Delivery Days Customer
    23
  • Application Supplier Note
    The predicted MW is 37kDa/86kDa/91kDa, while the observed MW by Western blot was 91kDa.
  • Applications
    Western Blot
  • Applications Supplier
    WB (1:500 - 1:2000) The predicted MW is 37kDa/86kDa/91kDa, while the observed MW by Western blot was 91kDa.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID54431
  • Target name
    DNAJC10
  • Target description
    DnaJ heat shock protein family (Hsp40) member C10
  • Target synonyms
    ERdj5, JPDI, MTHr, PDIA19, dnaJ homolog subfamily C member 10, DnaJ (Hsp40) homolog, subfamily C, member 10, ER-resident protein ERdj5, J-domain-containing protein disulfide isomerase-like protein, endoplasmic reticulum DNA J domain-containing protein 5, macrothioredoxin, protein disulfide isomerase family A, member 19
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of DNAJC10 using anti-DNAJC10 antibody (A07954-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human HELA whole cell lysates, Lane 2: human HEPG2 whole cell lysates, Lane 3: human U87 whole cell lysates, Lane 4: rat stomach tissue lysates, Lane 5: rat RH35 whole cell lysates, Lane 6: mouse stomach tissue lysates, Lane 7: mouse HEPA1-6 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DNAJC10 antigen affinity purified polyclonal antibody (Catalog # A07954-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for DNAJC10 at approximately 91KD. The expected band size for DNAJC10 is at 91KD.
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