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Western Blot analysis of LOVO cells using CSP Polyclonal Antibody
Western Blot analysis of LOVO cells using CSP Polyclonal Antibody
Western Blot analysis of LOVO cells using CSP Polyclonal Antibody

DNAJC5 Antibody

CSB-PA001802
Cusabio
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetDNAJC5
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Overview

  • Supplier
    Cusabio
  • Product Name
    DNAJC5 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID80331
  • Target name
    DNAJC5
  • Target description
    DnaJ heat shock protein family (Hsp40) member C5
  • Target synonyms
    CLN4, CLN4B, CSP, DNAJC5A, NCL, mir-941-2, mir-941-3, mir-941-4, mir-941-5, dnaJ homolog subfamily C member 5, DnaJ (Hsp40) homolog, subfamily C, member 5, ceroid-lipofuscinosis neuronal protein 4, cysteine string protein alpha
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9H3Z4
  • Protein Name
    DnaJ homolog subfamily C member 5
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of DNAJC5 using anti-DNAJC5 antibody (A04220-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DNAJC5 antigen affinity purified polyclonal antibody (Catalog # A04220-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for DNAJC5 at approximately 24 kDa. The expected band size for DNAJC5 is at 22 kDa.
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