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ProductsBack/DNAJC5 / CSP Antibody

DNAJC5 / CSP Antibody

LS-C497199
LifeSpan BioSciences
ApplicationsWestern Blot
Product group Antibodies
TargetDNAJC5
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    DNAJC5 / CSP Antibody
  • Delivery Days Customer
    23
  • Application Supplier Note
    The predicted MW is 18kDa/22kDa, while the observed MW by Western blot was 30kDa.
  • Applications
    Western Blot
  • Applications Supplier
    WB (1:1000 - 1:2000) The predicted MW is 18kDa/22kDa, while the observed MW by Western blot was 30kDa.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID80331
  • Target name
    DNAJC5
  • Target description
    DnaJ heat shock protein family (Hsp40) member C5
  • Target synonyms
    ceroid-lipofuscinosis neuronal protein 4; CLN4; CLN4B; CSP; cysteine string protein alpha; DnaJ (Hsp40) homolog, subfamily C, member 5; dnaJ homolog subfamily C member 5; DNAJC5A; mir-941-2; mir-941-3; mir-941-4; mir-941-5; NCL
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of DNAJC5 using anti-DNAJC5 antibody (A04220-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DNAJC5 antigen affinity purified polyclonal antibody (Catalog # A04220-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for DNAJC5 at approximately 24 kDa. The expected band size for DNAJC5 is at 22 kDa.
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