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ProductsBack/DNMT1 antibody [60B1220.1]
FACS (Intracellular staining) analysis of HeLa cells using GTX48548 DNMT1 antibody [60B1220.1]. Blue : Primary antibody Orange : isotype control Fixation : 4% PFA
FACS (Intracellular staining) analysis of HeLa cells using GTX48548 DNMT1 antibody [60B1220.1]. Blue : Primary antibody Orange : isotype control Fixation : 4% PFA
FACS (Intracellular staining) analysis of HeLa cells using GTX48548 DNMT1 antibody [60B1220.1]. Blue : Primary antibody Orange : isotype control Fixation : 4% PFA

DNMT1 antibody [60B1220.1]

GTX48548
GeneTex
ApplicationsFlow Cytometry, ImmunoFluorescence, ImmunoPrecipitation, Mass Spectrometry, Western Blot, ChIP Chromatin ImmunoPrecipitation, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
DownloadDatasheet
Product group Antibodies
TargetDNMT1
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Overview

  • Supplier
    GeneTex
  • Product Name
    DNMT1 antibody [60B1220.1]
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 0.1 - 0.5 microg/ml. ICC/IF: 1:10. IHC-P: 1:50 - 1:500. IP: 1:10 - 1:500. ChIP assay: 1:20 - 1:1000. IHC (Free Floating): 1:500. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Flow Cytometry, ImmunoFluorescence, ImmunoPrecipitation, Mass Spectrometry, Western Blot, ChIP Chromatin ImmunoPrecipitation, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    60B1220.1
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID1786
  • Target name
    DNMT1
  • Target description
    DNA methyltransferase 1
  • Target synonyms
    ADCADN; AIM; CXXC9; CXXC-type zinc finger protein 9; DNA (cytosine-5-)-methyltransferase 1; DNA (cytosine-5)-methyltransferase 1; DNA methyltransferase HsaI; DNA MTase HsaI; DNMT; HSN1E; m.HsaI; MCMT
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDP26358
  • Protein Name
    DNA (cytosine-5)-methyltransferase 1
  • Scientific Description
    DNA (cytosine-5-)-methyltransferase 1 has a role in the establishment and regulation of tissue-specific patterns of methylated cytosine residues. Aberrant methylation patterns are associated with certain human tumors and developmental abnormalities. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Dnmt1 using anti-Dnmt1 antibody (A00172-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human U-937 cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Dnmt1 antigen affinity purified polyclonal antibody (Catalog # A00172-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Dnmt1 at approximately 200KD. The expected band size for Dnmt1 is at 183KD.
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