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DNMT1 Rabbit pAb

A5495
ABclonal
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ELISA, ImmunoCytoChemistry
Product group Antibodies
ReactivityHuman
TargetDNMT1
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Overview

  • Supplier
    ABclonal
  • Product Name
    DNMT1 Rabbit pAb
  • Delivery Days Customer
    23
  • Applications
    ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ELISA, ImmunoCytoChemistry
  • Applications Supplier
    WB,IF/ICC,IP
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID1786
  • Target name
    DNMT1
  • Target description
    DNA methyltransferase 1
  • Target synonyms
    ADCADN, AIM, CXXC9, DNMT, HSN1E, MCMT, m.HsaI, DNA (cytosine-5)-methyltransferase 1, CXXC-type zinc finger protein 9, DNA (cytosine-5-)-methyltransferase 1, DNA MTase HsaI, DNA methyltransferase HsaI
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP26358
  • Protein Name
    DNA (cytosine-5)-methyltransferase 1
  • Reactivity
    Human
  • Reactivity Supplier Note
    Human
  • Storage Instruction
    -20°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of Dnmt1 using anti-Dnmt1 antibody (A00172-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human U-937 cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Dnmt1 antigen affinity purified polyclonal antibody (Catalog # A00172-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Dnmt1 at approximately 200KD. The expected band size for Dnmt1 is at 183KD.
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