Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Dopamine beta Hydroxylase antibody [HL1163] (GTX636467) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.
![Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membranes were blotted with Dopamine beta Hydroxylase antibody [HL1163] (GTX636467) diluted at 1:1000 and competitor's antibody (# Highly Cited Antibody) diluted at 1:200. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced. *The competitor is not affiliated with GeneTex and does not endorse this product.](https://www.genetex.com/upload/website/prouct_img/normal/GTX636467/GTX636467_44480_20220819_WB_competitor_watermark_22082402_433.webp "Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membranes were blotted with Dopamine beta Hydroxylase antibody [HL1163] (GTX636467) diluted at 1:1000 and competitor's antibody (# Highly Cited Antibody) diluted at 1:200. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced. *The competitor is not affiliated with GeneTex and does not endorse this product.")
![Dopamine beta Hydroxylase antibody [HL1163] detects Dopamine beta Hydroxylase protein at cytoplasm by immunofluorescent analysis. Sample: SH-SY5Y cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Dopamine beta Hydroxylase stained by Dopamine beta Hydroxylase antibody [HL1163] (GTX636467) diluted at 1:200. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000. Blue: Fluoroshield with DAPI (GTX30920). Scale bar= 10μm.](https://www.genetex.com/upload/website/prouct_img/normal/GTX636467/GTX636467_44480_20230210_ICC_IF_23021401_517.webp "Dopamine beta Hydroxylase antibody [HL1163] detects Dopamine beta Hydroxylase protein at cytoplasm by immunofluorescent analysis. Sample: SH-SY5Y cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Dopamine beta Hydroxylase stained by Dopamine beta Hydroxylase antibody [HL1163] (GTX636467) diluted at 1:200. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000. Blue: Fluoroshield with DAPI (GTX30920). Scale bar= 10μm.")
Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Dopamine beta Hydroxylase antibody [HL1163] (GTX636467) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.
Dopamine beta Hydroxylase antibody [HL1163]
GTX636467
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry
Product group Antibodies
ReactivityHuman
TargetDBH
Overview
- SupplierGeneTex
- Product NameDopamine beta Hydroxylase antibody [HL1163]
- Delivery Days Customer9
- Application Supplier NoteWB: 1:500-1:3000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone IDHL1163
- Concentration1 mg/ml
- ConjugateUnconjugated
- Gene ID1621
- Target nameDBH
- Target descriptiondopamine beta-hydroxylase
- Target synonymsDBM, ORTHYP1, dopamine beta-hydroxylase, dopamine beta-hydroxylase (dopamine beta-monooxygenase)
- HostRabbit
- IsotypeIgG
- Protein IDP09172
- Protein NameDopamine beta-hydroxylase
- Scientific DescriptionThe protein encoded by this gene is an oxidoreductase belonging to the copper type II, ascorbate-dependent monooxygenase family. The encoded protein, expressed in neuroscretory vesicles and chromaffin granules of the adrenal medulla, catalyzes the conversion of dopamine to norepinephrine, which functions as both a hormone and as the main neurotransmitter of the sympathetic nervous system. The enzyme encoded by this gene exists exists in both soluble and membrane-bound forms, depending on the absence or presence, respectively, of a signal peptide. Mutations in this gene cause dopamine beta-hydroxylate deficiency in human patients, characterized by deficits in autonomic and cardiovascular function, including hypotension and ptosis. Polymorphisms in this gene may play a role in a variety of psychiatric disorders. [provided by RefSeq, Aug 2017]
- ReactivityHuman
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203
Datasheet
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