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Western Blot analysis of HepG2 cells using DPYD Polyclonal Antibody
Western Blot analysis of HepG2 cells using DPYD Polyclonal Antibody
Western Blot analysis of HepG2 cells using DPYD Polyclonal Antibody

DPYD Antibody

CSB-PA005172
Cusabio
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetDPYD
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Overview

  • Supplier
    Cusabio
  • Product Name
    DPYD Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID1806
  • Target name
    DPYD
  • Target description
    dihydropyrimidine dehydrogenase
  • Target synonyms
    DHP, DHPDHASE, DPD, DYPD, dihydropyrimidine dehydrogenase [NADP(+)], dihydrothymine dehydrogenase, dihydrouracil dehydrogenase
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ12882
  • Protein Name
    Dihydropyrimidine dehydrogenase [NADP(+)]
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of DPYD using anti-DPYD antibody (A01749). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human SW620 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DPYD antigen affinity purified polyclonal antibody (Catalog # A01749) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for DPYD at approximately 111KD. The expected band size for DPYD is at 111KD.
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ReactivityHuman, Mouse, Rat
TargetDPYD
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TargetDPYD
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Bioss
DPYD Recombinant Antibody, AbBy Fluor-647 ConjugatedBSM-62352R-BF647
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry
ReactivityHuman
TargetDPYD
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Product group Antibodies
Biomatik
Dihydropyrimidine Dehydrogenase (DPYD) Polyclonal AntibodyCAU24845
ApplicationsImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityMouse, Porcine, Rat
TargetDPYD
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ReactivityHuman
TargetDPYD
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ReactivityHuman, Mouse, Rat
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Whole cell extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with DPYD antibody [N1N2], N-term (GTX113672) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Product group Antibodies
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DPYD antibody [N1N2], N-termGTX113672
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
ReactivityHuman
TargetDPYD
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Product group Antibodies
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Anti-DPYD AntibodyA98194
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ReactivityHuman, Mouse, Rat
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