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ProductsBack/DPYD Recombinant Antibody, AbBy Fluor-647 Conjugated

DPYD Recombinant Antibody, AbBy Fluor-647 Conjugated

BSM-62352R-BF647
Bioss
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry
Product group Antibodies
ReactivityHuman
TargetDPYD
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Overview

  • Supplier
    Bioss
  • Product Name
    DPYD Recombinant Antibody, AbBy Fluor-647 Conjugated
  • Delivery Days Customer
    16
  • Applications
    Flow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry
  • Applications Supplier
    WB(1:300-5000), FCM(1:20-100), IF(ICC)(1:50-200), IF()
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Conjugate
    Other Conjugate
  • Gene ID1806
  • Target name
    DPYD
  • Target description
    dihydropyrimidine dehydrogenase
  • Target synonyms
    DHP, DHPDHASE, DPD, DYPD, dihydropyrimidine dehydrogenase [NADP(+)], dihydrothymine dehydrogenase, dihydrouracil dehydrogenase
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ12882
  • Protein Name
    Dihydropyrimidine dehydrogenase [NADP(+)]
  • Reactivity
    Human
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of DPYD using anti-DPYD antibody (A01749). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human SW620 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DPYD antigen affinity purified polyclonal antibody (Catalog # A01749) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for DPYD at approximately 111KD. The expected band size for DPYD is at 111KD.
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Whole cell extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with DPYD antibody [N1N2], N-term (GTX113672) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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