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ProductsBack/DROSHA / RNASEN Antibody

DROSHA / RNASEN Antibody

LS-C830025
LifeSpan BioSciences
ApplicationsELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse
TargetDROSHA
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    DROSHA / RNASEN Antibody
  • Delivery Days Customer
    23
  • Applications
    ELISA, ImmunoHistoChemistry
  • Applications Supplier
    ELISA (1:5000 - 1:10000), IHC (1:30 - 1:150)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID29102
  • Target name
    DROSHA
  • Target description
    drosha ribonuclease III
  • Target synonyms
    ETOHI2, HSA242976, RANSE3L, RN3, RNASE3L, RNASEN, ribonuclease 3, RNase III, drosha, double-stranded RNA-specific endoribonuclease, nuclear RNase III Drosha, p241, putative protein p241 which interacts with transcription factor Sp1, putative ribonuclease III, ribonuclease type III, nuclear
  • Host
    Rabbit
  • Isotype
    IgG
  • Reactivity
    Human, Mouse
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with Drosha antibody [GT1178] (GTX09511) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.
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Figure 1. Western blot analysis of DROSHA using anti-DROSHA antibody (A00111-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human HEK293 whole cell lysates, Lane 3: human Jurkat whole cell lysates, Lane 4: human HepG2 whole cell lysates, Lane 5: human SW620 whole cell lysates, Lane 6: human K562 whole cell lysates, Lane 7: mouse Neuro-2a whole cell lysates, Lane 8: mouse Ana-1 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DROSHA antigen affinity purified polyclonal antibody (Catalog # A00111-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for DROSHA at approximately 200KD. The expected band size for DROSHA is at 159KD.
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