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WB analysis of HeLa cell lysate using GTX31901 DRP1 antibody. Working concentration : 1 microg/ml
WB analysis of HeLa cell lysate using GTX31901 DRP1 antibody. Working concentration : 1 microg/ml
WB analysis of HeLa cell lysate using GTX31901 DRP1 antibody. Working concentration : 1 microg/ml

DRP1 antibody

GTX31901
GeneTex
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry
Product group Antibodies
TargetDNM1L
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Overview

  • Supplier
    GeneTex
  • Product Name
    DRP1 antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1 - 2 microg/mL. ICC/IF: 5 microg/mL. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID10059
  • Target name
    DNM1L
  • Target description
    dynamin 1 like
  • Target synonyms
    DLP1, DRP1, DVLP, DYMPLE, EMPF, EMPF1, HDYNIV, OPA5, dynamin-1-like protein, Dnm1p/Vps1p-like protein, dynamin family member proline-rich carboxyl-terminal domain less, dynamin-like protein 4, dynamin-like protein IV, dynamin-related protein 1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO00429
  • Protein Name
    Dynamin-1-like protein
  • Scientific Description
    The protein encoded by this gene is a member of the dynamin superfamily of GTPases. Members of the dynamin-related subfamily, including the S. cerevisiae proteins Dnm1 and Vps1, contain the N-terminal tripartite GTPase domain but do not have the pleckstrin homology or proline-rich domains. This protein establishes mitochondrial morphology through a role in distributing mitochondrial tubules throughout the cytoplasm. The gene has 3 alternatively spliced transcripts encoding different isoforms. These transcripts are alternatively polyadenylated. [provided by RefSeq, Jul 2008]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

References

  • Chlamydia preserves the mitochondrial network necessary for replication via microRNA-dependent inhibition of fission. Chowdhury SR et al., 2017 Apr 3, J Cell Biol
    Read more
  • SIRT3 deacetylates FOXO3 to protect mitochondria against oxidative damage. Tseng AH et al., 2013 Oct, Free Radic Biol Med
    Read more

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Figure 1. Western blot analysis of DRP1/DNM1L using anti-DRP1/DNM1L antibody (A00556-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions. Lane 1: human Caco-2 whole cell lysates, Lane 2: human K562 whole cell lysates, Lane 3: human A549 whole cell lysates, Lane 4: rat heart tissue lysates, Lane 5: rat kidney tissue lysates, Lane 6: rat liver tissue lysates, Lane 7: rat brain tissue lysates, Lane 8: mouse HEPA1-6 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DRP1/DNM1L antigen affinity purified polyclonal antibody (Catalog # A00556-2) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for DRP1/DNM1L at approximately 82KD. The expected band size for DRP1/DNM1L is at 82KD.
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