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ProductsBack/DNM1L Polyclonal Antibody
Lane 1: Mouse Cerebrum tissue lysates; Lane 2: Mouse Cerebellum tissue lysates probed with DNM1L/DLP1 Polyclonal Antibody, Unconjugated (bs-4100R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: Mouse Cerebrum tissue lysates; Lane 2: Mouse Cerebellum tissue lysates probed with DNM1L/DLP1 Polyclonal Antibody, Unconjugated (bs-4100R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: Mouse Cerebrum tissue lysates; Lane 2: Mouse Cerebellum tissue lysates probed with DNM1L/DLP1 Polyclonal Antibody, Unconjugated (bs-4100R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

DNM1L Polyclonal Antibody

BS-4100R
Bioss Antibodies
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
DownloadDatasheet
Product group Antibodies
TargetDNM1L
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Overview

  • Supplier
    Bioss Antibodies
  • Product Name
    DLP1 Polyclonal Antibody
  • Delivery Days Customer
    16
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    WB(1:300-5000), ELISA(1:500-1000), IHC-P(1:200-400), IHC-F(1:100-500), IF(IHC-P)(1:50-200), IF(IHC-F)(1:50-200), IF(ICC)(1:50-200)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 ug/ul
  • Conjugate
    Unconjugated
  • Gene ID10059
  • Target name
    DNM1L
  • Target description
    dynamin 1 like
  • Target synonyms
    DLP1; Dnm1p/Vps1p-like protein; DRP1; DVLP; DYMPLE; dynamin family member proline-rich carboxyl-terminal domain less; dynamin-1-like protein; dynamin-like protein 4; dynamin-like protein IV; dynamin-related protein 1; EMPF; EMPF1; HDYNIV; OPA5
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO00429
  • Protein Name
    Dynamin-1-like protein
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

References

  • Hydrogen sulfide-induced oxidative stress leads to excessive mitochondrial fission to activate apoptosis in broiler myocardia. Wang S et al., 2019 Nov 15, Ecotoxicol Environ Saf
    Read more
  • Resveratrol improves mitochondrial function in the remnant kidney from 5/6 nephrectomized rats. Hui Y et al., 2017 May, Acta Histochem
    Read more
  • Connexin43 regulates high glucose-induced expression of fibronectin, ICAM-1 and TGF-beta1 via Nrf2/ARE pathway in glomerular mesangial cells. Chen Z et al., 2017 Jan, Free Radic Biol Med
    Read more

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Figure 1. Western blot analysis of DRP1/DNM1L using anti-DRP1/DNM1L antibody (A00556-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions. Lane 1: human Caco-2 whole cell lysates, Lane 2: human K562 whole cell lysates, Lane 3: human A549 whole cell lysates, Lane 4: rat heart tissue lysates, Lane 5: rat kidney tissue lysates, Lane 6: rat liver tissue lysates, Lane 7: rat brain tissue lysates, Lane 8: mouse HEPA1-6 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DRP1/DNM1L antigen affinity purified polyclonal antibody (Catalog # A00556-2) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for DRP1/DNM1L at approximately 82KD. The expected band size for DRP1/DNM1L is at 82KD.
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