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Western Blot analysis of Jurkat cells using dTMP Kinase Polyclonal Antibody
Western Blot analysis of Jurkat cells using dTMP Kinase Polyclonal Antibody
Western Blot analysis of Jurkat cells using dTMP Kinase Polyclonal Antibody

DTYMK Antibody

CSB-PA002214
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
TargetDTYMK
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Overview

  • Supplier
    Cusabio
  • Product Name
    DTYMK Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID1841
  • Target name
    DTYMK
  • Target description
    deoxythymidylate kinase
  • Target synonyms
    CDC8; deoxythymidylate kinase (thymidylate kinase); dTMP kinase; PP3731; thymidylate kinase; TMPK; TYMK
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP23919
  • Protein Name
    Thymidylate kinase
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of DTYMK using anti-DTYMK antibody (M12209-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A549 whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human PC-3 whole cell lysates, Lane 4: human U20S whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DTYMK antigen affinity purified monoclonal antibody (Catalog # M12209-1) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for DTYMK at approximately 24 kDa. The expected band size for DTYMK is at 24 kDa.
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