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Dual Specificity Phosphatase 3 (DUSP3) Polyclonal Antibody

CAU22351
Biomatik
ApplicationsWestern Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetDUSP3
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Overview

  • Supplier
    Biomatik
  • Product Name
    Dual Specificity Phosphatase 3 (DUSP3) Polyclonal Antibody
  • Delivery Days Customer
    12
  • Applications
    Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    WB; ICC; IHC-P; IHC-F; ELISA.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID1845
  • Target name
    DUSP3
  • Target description
    dual specificity phosphatase 3
  • Target synonyms
    VHR, dual specificity protein phosphatase 3, dual specificity protein phosphatase VHR, serine/threonine specific protein phosphatase, vaccinia H1-related phosphatase, vaccinia virus phosphatase VH1-related
  • Host
    Rabbit
  • Protein IDP51452
  • Protein Name
    Dual specificity protein phosphatase 3
  • Scientific Description
    The Dual Specificity Phosphatase 3 (DUSP3) Polyclonal Antibody (Species: Human) has been validated for the following applications: WB, ICC, IHC-P, IHC-F, ELISA.
  • Reactivity Supplier
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

FAQ

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Figure 1. Western blot analysis of DUSP3 using anti-DUSP3 antibody (A06135). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human CACO-2 whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: rat skeletal muscle tissue lysates, Lane 4: rat heart tissue lysates, Lane 5: mouse skeletal muscle tissue lysates, Lane 6: mouse heart tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DUSP3 antigen affinity purified polyclonal antibody (Catalog # A06135) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for DUSP3 at approximately 20 kDa. The expected band size for DUSP3 is at 20 kDa.
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